A MOLECULAR, ISOZYME AND MORPHOLOGICAL MAP OF THE BARLEY (HORDEUM-VULGARE) GENOME
A MOLECULAR, ISOZYME AND MORPHOLOGICAL MAP OF THE BARLEY (HORDEUM-VULGARE) GENOME
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DOI：
10.1007/bf00222660
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发表时间：
1993-07-01
影响因子：
5.4
通讯作者：
STEFFENSON, BJ
中科院分区：
文献类型：
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作者：
KLEINHOFS, A;KILIAN, A;STEFFENSON, BJ
构建了由295个基因座组成的大麦基因组图谱。这些座位包括152个DNA限制性片段长度多态性(RFLP)、114个基因组DNA RFLP、14个随机扩增多态性DNA(RAPD)、5个同工酶、2个形态标记、1个抗病标记和7个特异扩增多态(SAP)标记。RFLP鉴定的基因座包括63个用克隆的已知功能基因作为探针检测到的基因。这张地图覆盖了1250厘米，标记之间的平均距离为4.2厘米。染色体的遗传长度从124到223厘米不等，与它们的物理长度大致一致。着丝粒定位于除5号染色体外的所有大麦染色体上的几个标记。端粒区域定位于1、2、3号染色体的短(正)臂和7号染色体的长(负)臂。
A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven specific amplicon polymorphism (SAP) markers. The RFLP-identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosomes 1, 2 and 3 and the long (minus) arm of chromosomes 7.