Protective Effect of Circular RNA (CircRNA ) Ddx17 in Ovalbumin (OVA)-Induced Allergic Rhinitis (AR) Mice (Retracted Article)
Protective Effect of Circular RNA (CircRNA ) Ddx17 in Ovalbumin (OVA)-Induced Allergic Rhinitis (AR) Mice (Retracted Article)
复制标题
DOI：
10.12659/msm.919083
复制
发表时间：
2020-01-30
影响因子：
3.1
通讯作者：
Cao, Zhiwei
中科院分区：
文献类型：
--
作者：
Liu, Jiao;Cao, Zhiwei
背景：CircRNAs参与多种生物过程，特别是当它们作为miRNA的海绵时。因此，本研究在动物模型中研究了circddx17对变应性鼻炎（AR）的作用，并确定了参与该作用的miRNA。材料/方法：通过重复刺激卵清蛋白（OVA）建立AR模型。采用qPCR检测血浆mrna水平。使用RNase r评估CircDdx17的稳定性，通过生物信息学预测CircDdx17和miR-17-5p之间的相互作用，并通过双荧光素酶测定证实。记录患者摩擦、打喷嚏次数及病理变化，ELISA检测ova特异性IgE、肿瘤坏死因子(TNF)- α、白细胞介素(IL)-4、IL-5水平。结果：ova诱导的AR小鼠中circDdx17水平降低，miR-17-5p在小鼠脾细胞中与circDdx17相互作用。此外，circDdx17过表达可降低ova诱导AR小鼠miR-17-5p、ova特异性IgE、tnf - α、IL-4和IL-5的表达，以及摩擦和打喷嚏的频率，减轻其病理改变。结论：CircDdx17似乎对小鼠AR的进展具有保护作用，具体来说，过表达CircDdx17可以抑制miR-17-5p的表达，减轻AR的病情。因此，CircDdx17似乎是预防AR的一个很好的候选物。然而，CircDdx17 /miR-17-5p调控通路的详细机制需要进一步研究。
Background: CircRNAs are involved in multiple biological processes, especially when they act as sponges of miRNA. Thus, the present study investigated the effect of circDdxl7 on allergic rhinitis (AR) in an animal model, and determined the miRNA that was involved in this effect.Material/Methods: The AR model was created by repetitive stimulation of ovalbumin (OVA). The levels of mRNAs in plasma were determined by qPCR. CircDdx17 stability was assessed using RNase R. The interaction between circDdx17 and miR-17-5p was predicted by bioinformatics and confirmed by dual luciferase assay. Moreover, the frequencies of rubbing and sneezing and pathological changes were recorded, and OVA-specific IgE, tumor necrosis factor (TNF)-alpha, interleukin (IL)-4, and IL-5 levels were detected by ELISA.Results: Levels of circDdx17 were decreased in OVA-induced AR mice, and miR-17-5p interacted with circDdx17 in spleen cells derived from mice. Moreover, circDdx17 overexpression reduced the expression of miR-17-5p, OVA-specific IgE, TNF-alpha, IL-4, and IL-5, as well as the frequencies of rubbing and sneezing, and alleviated pathological changes in OVA-induced AR mice.Conclusions: CircDdx17 appears to have a protective effect on mice in the progression of AR. Specifically, overexpression of circDdx17 inhibited the expression of miR-17-5p and alleviated the condition of AR. Therefore, circDdx17 appears to be a good candidate for use in prevention of AR. However, the detailed mechanism underlying the circDdx17/miR-17-5p regulatory pathway requires further study.