REGULATION OF NEUROTROPHIN MESSENGER-RNA EXPRESSION IN THE RAT-BRAIN BY GLUCOCORTICOIDS
REGULATION OF NEUROTROPHIN MESSENGER-RNA EXPRESSION IN THE RAT-BRAIN BY GLUCOCORTICOIDS
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DOI：
10.1111/j.1460-9568.1992.tb00888.x
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发表时间：
1992-05-01
影响因子：
3.4
通讯作者：
PERSSON, H
中科院分区：
文献类型：
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作者：
BARBANY, G;PERSSON, H
采用北方印迹法检测糖皮质激素对大鼠大脑皮层和海马神经营养因子mRNA表达的影响。结果显示，肾上腺切除后3天，这两个区域的神经生长因子（NGF）、脑源性神经营养因子（BDNF）和神经营养素-3（NT-3）的mRNA水平显著降低。在给予地塞米松替代的肾上腺切除动物中，三种神经营养因子的mRNA水平恢复到对照水平。还检查了对完整动物腹膜内给予单剂量地塞米松（5 mg/kg）对神经营养因子表达的影响。在注射后的第一个4小时内，NGF和NT-3 mRNA分别增加了2.5倍和1.4倍。增加后降低，注射后24和48 h的水平约为对照的50%。与此相反，BDNF mRNA的水平在注射后的第一个10小时内没有变化，但在注射后48小时下降到对照的70%。这些数据表明，糖皮质激素调节神经营养因子mRNA的表达在皮层和海马，并进一步表明，已知的影响，糖皮质激素对神经元的存活在大脑中可能是由于在大脑中的神经营养因子的水平的变化。
Northern blot analysis was used to examine the effects of glucocorticoids on neurotrophin mRNA expression in the rat cerebral cortex and hippocampus. The results show that 3 days after adrenalectomy the mRNA levels for nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) decreased significantly in both these regions. In adrenalectomized animals given dexamethasone replacement the mRNA levels for the three neurotrophins were restored to control levels. The effect of a single dose of dexamethasone (5 mg/kg) administered i.p. to intact animals on the expression of neurotrophins was also examined. NGF and NT-3 mRNAs showed a 2.5-fold and a 1.4-fold increase, respectively, during the first 4 h after the injection. The increase was followed by a decrease, with levels approximately 50% of control 24 and 48 h after the injection. In contrast, the level of BDNF mRNA did not change during the first 10 h after the injection, but decreased to 70% of control 48 h after the injection. These data indicate that glucocorticoids regulate neurotrophin mRNA expression both in the cortex and in the hippocampus, and suggest further that the known effects of glucocorticoids on neuronal survival in the brain could be due to changes in the levels of neurotrophins in the brain.