Rhubarb-Evoke Mucus Secretion through Aggregation and Degranulation of Mast Cell in the Colon of Rat: In vivo and ex vivo studies
Rhubarb-Evoke Mucus Secretion through Aggregation and Degranulation of Mast Cell in the Colon of Rat: In vivo and ex vivo studies
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DOI：
10.1038/s41598-019-55937-7
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发表时间：
2019-12
影响因子：
4.6
通讯作者：
Di Wu;X. Xue;Chen-chen Gao;Yuehong Liu;Tiantian Wang;Lisheng Li;X. Tong;F. Li;Jingdong Xu
中科院分区：
文献类型：
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作者：
Di Wu;X. Xue;Chen-chen Gao;Yuehong Liu;Tiantian Wang;Lisheng Li;X. Tong;F. Li;Jingdong Xu
在中国，大黄常用于治疗便秘，因为它具有促进肠道运动和优化粪便含水量的功能。但其分泌粘液的机制尚不清楚。本研究旨在探讨肥大细胞和肠神经元在大黄提取物（RE）诱导的大鼠结肠粘液分泌中的作用。免疫荧光法检测组胺受体。采用Western blotting和3,3′-二氨基联苯胺（DAB）检测肥大细胞活化后的含量变化。采用PAS/AB染色法测定结肠杯状细胞（GCs）的变化。肠灌注系统与布拉德福德蛋白测定试剂盒直接估计体外分泌。ELISA法检测细胞因子。本研究的纵向方面表明，在施用不同剂量的RE后，粪便颗粒的数量和含水量增加，并伴有肥大细胞积聚，并且在剂量为3和6 g/kg时增加干扰素(IFN) -γ的含量或降低白细胞介素(IL) - 10的水平。用肥大细胞稳定剂酮替芬预处理，可部分抑制re诱导的粘液分泌。此外，RE诱导了结肠组织中乙酰胆碱和粘蛋白-2的释放以及粪便中的组胺水平。结果提示，RE诱导的结肠粘液分泌涉及肥大细胞活化和部分细胞因子。
Rhubarb is commonly used to treat constipation in China for its function of promoting intestinal movement and optimum water content in feces. However, its mechanism of mucus secretion is vague. The aim of the study is to investigate the role of mast cells and enteric neurons in rhubarb extract (RE)-induced mucus secretion in the rat colon. Immunofluorescence was used to detect histamine receptors. Western blotting and 3,3′-diaminobenzidine (DAB) were applied to explore the content changes of mast cells activation. The changes in colonic goblet cells (GCs) were determined by means of PAS/AB staining. An intestinal perfusion system with a Bradford protein assay kit was directly to estimatein vitrosecretion. And the cytokines were investigated with ELISA. The longitudinal aspect of this study indicate that the number and water content of faecal pellets were enhanced after the administration of different doses of RE accompanied by mast cells accumulated and increased the content of interferon (IFN) -γ or decreased the levels of interleukin (IL) −10 at doses of 3 and 6 g/kg. Pretreatment with ketotifen, mast cell stabilizer, had partially inhibited on RE-induced mucus secretion. Furthermore, RE induced the release of acetylcholine and mucin-2 in the colonic tissue and the histamine levels from the faeces. The results suggest that RE induced colonic mucus secretion involves mast cell activation and some cytokine.