Analytical and toxicological evaluation of flavor chemicals in electronic cigarette refill fluids
Analytical and toxicological evaluation of flavor chemicals in electronic cigarette refill fluids
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DOI：
10.1038/s41598-018-25575-6
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发表时间：
2018-05-29
影响因子：
4.6
通讯作者：
Talbot, Prue
中科院分区：
文献类型：
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作者：
Behar, Rachel Z.;Luo, Wentai;Talbot, Prue
数以千计的电子烟再填充流体是可商购的。尼古丁和溶剂的浓度，但不是香料化学品，通常在产品标签上披露。本研究的目的是确定和量化39个商业再填充液中的风味化学物质，这些化学物质之前曾进行过毒性评价。12种浓度>= 1 mg/ml的风味化学物质被鉴定：肉桂醛、薄荷醇、苯甲醇、香草醛、丁香酚、对茴香醛、肉桂酸乙酯、麦芽酚、乙基麦芽酚、三醋精、苯甲醛和薄荷酮。将这些风味化学品转移到在3V和5V下制备的气溶胶中是有效的（平均转移= 98%）。我们生产了实验室制造的含有每种香料化学品真实标准品的补充液，并使用罐盒Mod设备分析了在3V和5V下产生的气溶胶的毒性。在我们的样品中，超过50%的填充液含有高浓度的香料化学物质，这些化学物质在产生细胞毒性的浓度下有效地转移到气溶胶中。当用两种类型的人类肺细胞进行测试时，在5V下制造的气溶胶通常比在3V下制造的气溶胶更具毒性。这些数据对于消费者、医生、公共卫生官员和监管机构在讨论与电子烟产品中的香料化学物质有关的潜在健康问题时将是有价值的。
Thousands of electronic cigarette refill fluids are commercially available. The concentrations of nicotine and the solvents, but not the flavor chemicals, are often disclosed on product labels. The purpose of this study was to identify and quantify flavor chemicals in 39 commercial refill fluids that were previously evaluated for toxicity. Twelve flavor chemicals were identified with concentrations >= 1 mg/ml: cinnamaldehyde, menthol, benzyl alcohol, vanillin, eugenol, p-anisaldehyde, ethyl cinnamate, maltol, ethyl maltol, triacetin, benzaldehyde, and menthone. Transfer of these flavor chemicals into aerosols made at 3V and 5V was efficient (mean transfer = 98%). We produced lab-made refill fluids containing authentic standards of each flavor chemical and analyzed the toxicity of their aerosols produced at 3V and 5V using a tank Box Mod device. Over 50% of the refill fluids in our sample contained high concentrations of flavor chemicals that transferred efficiently to aerosols at concentrations that produce cytotoxicity. When tested with two types of human lung cells, the aerosols made at 5V were generally more toxic than those made at 3V. These data will be valuable for consumers, physicians, public health officials, and regulatory agencies when discussing potential health concerns relating to flavor chemicals in electronic cigarette products.