Plant growth-promoting rhizobacteria systemically protect Arabidopsis thaliana against Cucumber mosaic virus by a salicylic acid and NPR1-independent and jasmonic acid-dependent signaling pathway
Plant growth-promoting rhizobacteria systemically protect Arabidopsis thaliana against Cucumber mosaic virus by a salicylic acid and NPR1-independent and jasmonic acid-dependent signaling pathway
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DOI：
10.1111/j.1365-313x.2004.02142.x
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发表时间：
2004-08-01
期刊：
影响因子：
7.2
通讯作者：
Kloepper, JW
中科院分区：
文献类型：
--
作者：
Ryu, CM;Murphy, JF;Kloepper, JW
用促生长根瘤菌（PGPR）、粘质Serattia marcescens菌株90-166和短芽孢杆菌（Bacillus pumilus）菌株SE34处理拟南芥生态型哥伦比亚植株（Col-0），可显著降低黄瓜花叶病毒（CMV）感染症状的严重程度。在某些情况下，CMV的积累在系统感染的叶片中也显著减少。利用突变株、转基因和突变拟南芥株系，确定了菌株90-166诱导CMV抗性的信号转导途径。用菌株90-166和SE34处理NahG植株，症状严重程度降低，表明抗性不需要水杨酸（SA）。菌株90-166在限铁条件下自然产生SA。用sa缺乏突变体90-166-1441处理过的col0和NahG植株的CMV症状严重程度显著降低，col0植株的病毒积累减少。另一个PGPR突变体90-166-2882在Col-0和NahG植物中导致疾病严重程度降低。在一项时间过程研究中，菌株90-166在接种后7天（dpi）减少了病毒在col0植株未接种叶片上的积累，而在接种后14天和21天（dpi）则没有。菌株90-166处理的NahG和npr1-1植株在7和14 dpi时病毒量减少，而在21 dpi时病毒量没有减少。相比之下，菌株90-166处理的fad3-2、fad7-2、fad8植株的CMV积累没有减少。这些数据表明，菌株90-166对拟南芥CMV的保护遵循一个独立于SA和NPR1的病毒保护信号通路，但依赖于茉莉酸。
Arabidopsis thaliana ecotype Columbia plants (Col-0) treated with plant growth-promoting rhizobacteria (PGPR) Serattia marcescens strain 90-166 and Bacillus pumilus strain SE34 had significantly reduced symptom severity by Cucumber mosaic virus (CMV). In some cases, CMV accumulation was also significantly reduced in systemically infected leaves. The signal transduction pathway(s) associated with induced resistance against CMV by strain 90-166 was determined using mutant strains and transgenic and mutant Arabidopsis lines. NahG plants treated with strains 90-166 and SE34 had reduced symptom severity indicating that the resistance did not require salicylic acid (SA). Strain 90-166 naturally produces SA under iron-limited conditions. Col-0 and NahG plants treated with the SA-deficient mutant, 90-166-1441, had significantly reduced CMV symptom severity with reduced virus accumulation in Col-0 plants. Another PGPR mutant, 90-166-2882, caused reduced disease severity in Col-0 and NahG plants. In a time course study, strain 90-166 reduced virus accumulation at 7 but not at 14 and 21 days post-inoculation (dpi) on the non-inoculated leaves of Col-0 plants. NahG and npr1-1 plants treated with strain 90-166 had reduced amounts of virus at 7 and 14 dpi but not at 21 dpi. In contrast, no decrease in CMV accumulation occurred in strain 90-166-treated fad3-2 fad7-2 fad8 plants. These data indicate that the protection of Arabidopsis against CMV by strain 90-166 follows a signaling pathway for virus protection that is independent of SA and NPR1, but dependent on jasmonic acid.