MEASUREMENT OF FOREARM OXYGEN-CONSUMPTION - ROLE OF HEATING THE CONTRALATERAL HAND
MEASUREMENT OF FOREARM OXYGEN-CONSUMPTION - ROLE OF HEATING THE CONTRALATERAL HAND
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DOI：
10.1152/ajpendo.1988.255.4.e572
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发表时间：
1988-10-01
影响因子：
--
通讯作者：
CHRISTENSEN, NJ
中科院分区：
文献类型：
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作者：
ASTRUP, A;SIMONSEN, L;CHRISTENSEN, NJ
广泛应用于骨骼肌代谢研究的经典前臂技术需要动脉插管。为了避免动脉穿孔，通过局部加热使对侧手部静脉的血液动脉化变得越来越常见。关于骨骼肌在葡萄糖诱导的产热中的作用，这一修饰和经典方法产生了相互矛盾的结果。在口服葡萄糖负荷前后检测对前臂循环和加热对侧手的代谢的影响。结果表明，对侧加热增加了皮下血流量，减少了骨骼肌血流量。这有利于浅静脉血和深静脉血的混合。对侧加热增加了深静脉血氧饱和度，并消除了在对照实验中观察到的葡萄糖引起的氧消耗的显著增加。加热使直肠温度升高0.6度。C，血浆去甲肾上腺素水平较对照组升高。本研究解释了关于葡萄糖诱导骨骼肌产热的相互矛盾的报道，并警告使用前臂技术时不要加热对侧手。
The classical forearm technique widely used for studies of skeletal muscle metabolism requires arterial cannulation. To avoid arterial puncture it is becoming more common to arterialize blood from a contralateral hand vein by local heating. This modification and the classical method have produced contradictory results regarding the contribution of skeletal muscle to glucose-induced thermogenesis. The effect on forearm circulation and the metabolism of heating the contralateral hand was examined before and after an oral glucose load. The results suggest that contralateral heating increases subcutaneous blood flow and decreases skeletal muscle blood flow. This facilitates mixing of superficial blood with deep venous blood. Contralateral heating increased deep venous oxygen saturation and abolished the pronounced glucose-induced increase in oxygen consumption observed in the control experiments after glucose. Heating increased rectal temperature by 0.6.degree. C, and plasma norepinephrine levels were increased compared with the control experiments. The present study explains the conflicting reports on glucose-induced thermogenesis in skeletal muscle and warns against heating the contralateral hand when using the forearm technique.