Phosphodiesteratic breakdown of endogenous polyphosphoinositides in nerve ending membranes.
Phosphodiesteratic breakdown of endogenous polyphosphoinositides in nerve ending membranes.
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DOI：
10.1016/0006-291x(83)91705-9
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发表时间：
1983-05
影响因子：
3.1
通讯作者：
L. A. A. Rooijen-L.-A.-A.-Rooijen-32100603;E. Seguin;B. Agranoff
中科院分区：
文献类型：
--
作者：
L. A. A. Rooijen-L.-A.-A.-Rooijen-32100603;E. Seguin;B. Agranoff
When a membrane preparation, obtained by freezing and thawing nerve endings labeled by preincubation with32Pi, is incubated in the presence of millimolar Ca2+, there is a rapid and selective loss of label from the polyphosphoinositides and a concomitant increase in labeled inositol di- and triphosphates recovered. When the membranes are not prelabeled and are exposed to [γ-32P]ATP under similar conditions, phosphatidate labeling is enhanced, indicating increased availability of diacylglycerol. These observations provide evidence for the presence of membrane-bound, Ca2+-stimulated phosphodiesterase activity (phospholipase C) acting on endogenous polyphosphoinositides. The implications of these findings are discussed in respect to the “phosphatidylinositol” cycle.
