渦鞭毛藻Prorocentrum limaの毒の主成分PL toxin-IIの単離と同定
渦鞭毛藻Prorocentrum limaの毒の主成分PL toxin-IIの単離と同定
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DOI：
10.2331/suisan.48.69
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发表时间：
1982-01
影响因子：
0.4
通讯作者：
安孝 村上;泰克 大島;健児 安元
中科院分区：
文献类型：
--
作者：
安孝 村上;泰克 大島;健児 安元
从底栖甲藻利马原甲藻（Prorocentrum lima）中分离到两种乙醚可溶性毒素（PL toxin-I、II）和一种正丁醇可溶性毒素（PL toxin-III）。主要毒性成分PL毒素-II通过几步柱色谱纯化，得到无色晶体：mp. 156-158°C，[α] 20 D +23°物理和化学数据表明分子式为C44 H68 O 13，分子中有四个羟基，一个羧基，七个醚键，三个烯烃和五个甲基。进一步检查其性质并与真实样品进行比较，确定PL毒素-II与冈田酸相同，冈田酸最近作为两种海绵Halichondria okadai和H.黑素血症
Two diethyl ether soluble toxins (PL toxin-I, II) and one 1-butanol soluble toxin (PL toxin-III) were isolated from a benthic marine dinoflagellate Prorocentrum lima. The major toxic component, PL toxin-II, was purified by several steps of column chromatography to yield colorless crystals: mp. 156-158°C, [α]20D+23° Physical and chemical data pointed to a molecular formula C44H68O13 having four hydroxyls, one carboxyl, seven ether linkages, three olefins, and five methyls in the molecule. A further examination of its properties and a comparison with an authentic specimen established that PL toxin-II is identical with okadaic acid, recently isolated as a cytotoxic component of two sponges Halichondria okadai and H. melanodocia.