The cyanobacterial homologue of the RNA chaperone Hfq is essential for motility of Synechocystis sp PCC 6803
The cyanobacterial homologue of the RNA chaperone Hfq is essential for motility of Synechocystis sp PCC 6803
复制标题
DOI：
10.1099/mic.0.2008/020222-0
复制
发表时间：
2008-10-01
期刊：
影响因子：
2.8
通讯作者：
Wilde, Annegret
中科院分区：
文献类型：
--
作者：
Dienst, Dennis;Duehring, Ulf;Wilde, Annegret
ssr3341位点先前被认为编码蓝藻胞杆菌菌株PCC 6803中RNA伴侣蛋白Hfq的同源物。该基因的插入失活导致突变体不能自然转化，与野生型相比表现出非光致表型。运动能力的丧失被野生型基因的重新引入所补充，这与细胞表面上IV型菌毛的重建有关。微阵列分析显示，与野生型细胞相比，敲除突变体中有一小部分基因的转录水平急剧降低。在受影响最强烈的基因中，slr1667、slr1668、slr2015、slr2016和slr2018尤为突出，因为它们属于两个先前被证明参与运动的操纵子，由cAMP受体蛋白SYCRP1控制。这表明cAMP信号传导、运动和可能参与rna调控之间存在联系。这被认为是第一个证明Hfq同源物在蓝藻中的功能作用的报告，建立了一个控制运动的新因素。
The ssr3341 locus was previously suggested to encode an orthologue of the RNA chaperone Hfq in the cyanobacterium Synechocystis sp. strain PCC 6803. Insertional inactivation of this gene resulted in a mutant that was not naturally transformable and exhibited a non-phototactic phenotype compared with the wild-type. The loss of motility was complemented by reintroduction of the wild-type gene, correlated with the re-establishment of type IV pili on the cell surface. Microarray analyses revealed a small set of genes with drastically reduced transcript levels in the knockout mutant compared with the wild-type cells. Among the most strongly affected genes, slr1667, slr1668, slr2015, slr2016 and slr2018 stood out, as they belong to two operons that had previously been shown to be involved in motility, controlled by the cAMP receptor protein SYCRP1. This suggests a link between cAMP signalling, motility and possibly the involvement of RNA-based regulation. This is believed to be the first report demonstrating a functional role of an Hfq orthologue in cyanobacteria, establishing a new factor in the control of motility.