NUCLEOTIDE-SEQUENCE OF THE GENE CODING FOR THE MAJOR PROTEIN OF HEPATITIS-B VIRUS SURFACE-ANTIGEN
NUCLEOTIDE-SEQUENCE OF THE GENE CODING FOR THE MAJOR PROTEIN OF HEPATITIS-B VIRUS SURFACE-ANTIGEN
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DOI：
10.1038/280815a0
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发表时间：
1979-01-01
期刊：
影响因子：
64.8
通讯作者：
RUTTER, WJ
中科院分区：
文献类型：
--
作者：
VALENZUELA, P;GRAY, P;RUTTER, WJ
使用质粒载体将从B型肝炎病毒Dane颗粒中提取的DNA克隆到细菌[大肠杆菌]中。用限制性内切酶分析了全长克隆，并报道了编码表面抗原基因的克隆B型肝炎病毒DNA的892个碱基对片段的核苷酸序列。从DNA推导的氨基酸序列表明，表面抗原是由226个氨基酸组成的蛋白质，MW为25，398。编码这种蛋白质的基因部分显然不含间插序列。
DNA extracted from hepatitis B virus Dane particles was cloned in bacteria [Escherichia coli] using a plasmid vector. A full-length clone has been examined by restriction endonuclease analysis, and the nucleotide sequence of an 892-base pair fragment from cloned hepatitis B viral DNA encoding the surface antigen gene is reported. The amino acid sequence deduced from the DNA indicates that the surface antigen is a protein consisting of 226 amino acids and with a MW of 25,398. The portion of the gene coding for this protein apparently contains no intervening sequences.