The effects of Lawsonia intracellularis, Salmonella enterica serovar Typhimurium and co-infection on IL-8 and TNFα expression in IPEC-J2 cells
The effects of Lawsonia intracellularis, Salmonella enterica serovar Typhimurium and co-infection on IL-8 and TNFα expression in IPEC-J2 cells
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DOI：
10.1016/j.vetmic.2019.02.036
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发表时间：
2019-04-01
影响因子：
3.3
通讯作者：
Isaacson, Richard E.
中科院分区：
文献类型：
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作者：
Leite, Fernando L.;Vasquez, Erika;Isaacson, Richard E.
胞内劳森氏菌是猪最重要的肠道病原体之一，已被证明是肠道沙门氏菌脱落增加的危险因素。S.肠道血清型鼠伤寒（Typhimurium）除了是猪的重要病原体之外，也仍然是全世界食源性疾病的最常见原因之一。炎症和IL 8、TNF α的表达是S.鼠伤寒感染。然而，L intracellularis对这些细胞因子的表达肠上皮细胞，这两种病原体在感染过程中占据的利基的影响，是知之甚少。在这项研究中，我们比较了细胞因子基因表达之间的单一和双重感染的IPEC-J2细胞，非转化的猪肠上皮细胞系。结果表明，L.细胞内感染导致IL 8和TNF α的表达增加，并对它们在共感染中的表达具有累加效应。炎性细胞因子表达的增加可能是L. intracellularis有利于S.鼠伤寒感染。
Lawsonia intracellularis is among the most important enteric pathogens of swine and has been shown to be a risk factor for increased Salmonella enterica shedding. S. enterica serovar Typhimurium, in addition to being a significant pathogen of swine, also remains one of the most common causes of foodbome illness worldwide. Inflammation and the expression of IL8 and TNF alpha are an important process in the establishment of S. Typhimurium infection. Yet the effect of L intracellularis on the expression of these cytokines by enterocytes, the niche both pathogens occupy during infection, is poorly understood. In this study we compared cytokine gene expression between singly and dually infected IPEC-J2 cells, a non-transformed porcine enterocyte cell line. Our results show that L. intracellularis leads to increased expression of IL8 and TNF alpha and has an additive effect on their expression in co-infection. The increase in expression of inflammatory cytokines may be one mechanism by which L. intracellularis favors S. Typhimurium infection.