Light-scattering study of depolymerization kinetics of sickle hemoglobin polymers inside single erythrocytes.
Light-scattering study of depolymerization kinetics of sickle hemoglobin polymers inside single erythrocytes.
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DOI：
10.1073/pnas.83.2.352
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发表时间：
1986
影响因子：
11.1
通讯作者：
J. Peetermans;I. Nishio;S. Ohnishi;T. Tanaka
中科院分区：
文献类型：
--
作者：
J. Peetermans;I. Nishio;S. Ohnishi;T. Tanaka
利用显微镜激光光散射谱，直接观察了单个镰刀状红细胞缓慢复氧时，血红蛋白S聚集体解聚的时间过程。根据单个细胞散射的光强的相关函数，我们确定了平均扩散系数以及具有细胞内迁移率的血红蛋白聚集体的比例。用单细胞吸收分光光度法测同一细胞的血氧饱和度。结合这些技术得到的结果和细胞形态的信息，我们提出了单个红细胞内血红蛋白解聚过程的模型，当它们在复氧时从完全病态转变为正常的双凹形状。
The time course of intracellular depolymerization of hemoglobin S aggregates is directly observed by using microscope laser light-scattering spectroscopy in single sickle erythrocytes upon slow reoxygenation. From the correlation functions of the light intensity scattered from a single cell, we determine the average diffusion coefficient as well as the fraction of hemoglobin aggregates that have intracellular mobility. The oxygen saturation of the hemoglobin of the same cell is measured by single-cell absorption spectrophotometry. Combining the results obtained with these techniques and information on the cellular morphology, we propose a model for the depolymerization process of hemoglobin inside single erythrocytes as they transform from the fully sickled to the normal biconcave shape upon reoxygenation.