Germ cells and fatty acids induce translocation of CD36 scavenger receptor to the plasma membrane of Sertoli cells
Germ cells and fatty acids induce translocation of CD36 scavenger receptor to the plasma membrane of Sertoli cells
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DOI：
10.1242/jcs.02430
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发表时间：
2005-07-15
影响因子：
4
通讯作者：
Vidal, F
中科院分区：
文献类型：
--
作者：
Gillot, I;Jehl-Pietri, C;Vidal, F
CD36清道夫受体参与了脂肪酸的摄取和运输，以及巨噬细胞的吞噬过程。我们发现CD36蛋白在生精上皮中的支持细胞中表达，主要发生在吞噬作用的阶段。使用Sertoli来源的细胞系，我们发现生殖细胞和残余体的加入会触发CD36从细胞质到细胞膜的重新定位，而乳胶珠不会。此外，支持细胞吞噬生殖细胞。而不是乳胶珠，由于培养基中存在脂肪酸而减少。在睾丸中，CD36在吞噬和脂质循环中发挥着关键作用，以维持成熟精子的持续产生。
The CD36 scavenger receptor is involved in the uptake and transport of fatty acids, as well as the phagocytosis process in macrophages. We show here that the CD36 protein is expressed by Sertoli cells in the seminiferous epithelium, mainly during the stages where phagocytosis takes place. Using a Sertoli-derived cell line, we show that addition of germ cells and residual bodies triggers a re-localization of CD36 from the cytoplasm to the plasma membrane of the cells, while latex beads do not. Moreover, Sertoli cell phagocytosis of germ cells. but not of latex beads, is reduced by the presence of fatty acids in the culture medium. In the testis, CD36 plays a key role in both phagocytosis and lipid recycling, for constant production of mature spermatozoa.