The THAP domain:: a novel protein motif with similarity to the DNA-binding domain of P element transposase
The THAP domain:: a novel protein motif with similarity to the DNA-binding domain of P element transposase
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DOI：
10.1016/s0968-0004(02)00013-0
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发表时间：
2003-02-01
影响因子：
13.8
通讯作者：
Girard, JP
中科院分区：
文献类型：
--
作者：
Roussigne, M;Kossida, S;Girard, JP
我们已经确定了一个新的进化保守的蛋白质基序--命名为TAP结构域--它定义了一个新的细胞因子家族。我们发现TAP结构域与果蝇P元件转座酶的位点特异性DNA结合域(DBD)具有显著的相似性，包括相似的大小、N-末端位置以及定义TAP基序的残基的保守性，例如C2CH签名(Cys-xaa(2-4)-Cys-xaa(35-50)-Cys-xaa(2)-His)。我们的结果表明，TAP结构域是一种新的果蝇P元件转座酶，包括相似的大小、N-末端位置和定义TAP基序的残基的保守性，例如C2CH信号(Cys-xaa(2-4)-Cys-xaa(35-50)-Cys-xaa(2)-His)。我们的结果表明，TAP结构域是一个新的DBD例子，它在细胞蛋白和移动基因组寄生虫的转座酶之间共享。
We have identified a novel evolutionarily conserved protein motif - designated the THAP domain - that defines a new family of cellular factors. We have found that the THAP domain presents striking similarities with the site-specific DNA-binding domain (DBD) of Drosophila P element transposase, including a similar size, N-terminal location, and conservation of the residues that define the THAP motif, such as the C2CH signature (Cys-Xaa(2-4)-CYS-Xaa(35-50)-Cys-Xaa(2)-His). Our results suggest that the THAP domain is a novel Drosophila P element transposase, including a similar size, N-terminal location, and conservation of the residues that define the THAP motif, such as the C2CH signature (Cys-Xaa(2-4)-CYS-Xaa(35-50)-Cys-Xaa(2)-His). Our results suggest that the THAP domain is a novel example of a DBD that is shared between cellular proteins and transposases from mobile genomic parasites.