SEQUENCE AND GENOME ORGANIZATION OF BORNA-DISEASE VIRUS
SEQUENCE AND GENOME ORGANIZATION OF BORNA-DISEASE VIRUS
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DOI：
10.1128/jvi.68.3.1382-1396.1994
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发表时间：
1994-03-01
影响因子：
5.4
通讯作者：
DELATORRE, JC
中科院分区：
文献类型：
--
作者：
CUBITT, B;OLDSTONE, C;DELATORRE, JC
我们以前已经证明，博尔纳病病毒（BDV）有一个负的非节段单链（NNS）RNA基因组，在感染细胞的细胞核中复制。本文首次报道了BDV基因组的克隆和全序列。我们的研究结果表明，BDV的基因组结构与单负加病毒目的其他成员相似。我们已经确定了五个主要的开放阅读框架（ORF）。最大的ORF V位于BDV基因组中最靠近5'端，并且基于与其它NNS-RNA病毒聚合酶的强同源性，是L-蛋白家族的成员。顺反子间区域的长度和核苷酸组成不同，并含有推定的转录起始和终止信号。BDV非翻译的3'和5' RNA序列类似于其他NNS-RNA病毒的序列。使用一组重叠的探针在BDV基因组，我们确定了9个体内合成的物种的多聚腺苷酸化的亚基因组RNA互补的负链RNA基因组，包括单顺反子转录对应的ORF I，II和IV，以及6个多顺反子多聚腺苷酸化的BDV RNA。有趣的是，虽然ORFs III和V在多顺反子转录本中检测到，但它们相应的单顺反子转录本未检测到。我们的数据表明，BDV是一个成员的单负加病毒目，特别是与家庭弹状病毒。然而，与其他NNS-RNA动物病毒相反，BDV复制和转录发生在感染细胞的细胞核中。这些发现表明BDV和植物弹状病毒之间可能存在关系，弹状病毒也在细胞核中复制和转录。
We have previously demonstrated that Borna disease virus (BDV) has a negative nonsegmented single-stranded (NNS) RNA genome that replicates in the nucleus of infected cells. Here we report for the first time the cloning and complete sequence of the BDV genome. Our results revealed that BDV has a genomic organization similar to that of other members of the Mononegavirales order. We have identified five main open reading frames (ORFs). The largest ORF, V, is located closest to the 5' end in the BDV genome and, on the basis of strong homology with other NNS-RNA virus polymerases, is a member of the L-protein family. The intercistronic regions vary in length and nucleotide composition and contain putative transcriptional start and stop signals. BDV untranslated 3' and 5' RNA sequences resemble those of other NNS-RNA viruses. Using a set of overlapping probes across the BDV genome, we identified nine in vivo synthesized species of polyadenylated subgenomic RNAs complementary to the negative-strand RNA genome, including monocistronic transcripts corresponding to ORFs I, II, and IV, as well as six polycistronic polyadenylated BDV RNAs. Interestingly, although ORFs III and V were detected within polycistronic transcripts, their corresponding monocistronic transcripts were not detected. Our data indicate that BDV is a member of the Mononegavirales, specially related to the family Rhabdoviridae. However, in contrast to the rest of the NNS-RNA animal viruses, BDV replication and transcription occur in the nucleus of infected cells. These findings suggest a possible relationship between BDV and the plant rhabdoviruses, which also replicate and transcribe in the nucleus.