Cardioprotective Effects of Morroniside in Rats Following Acute Myocardial Infarction
Cardioprotective Effects of Morroniside in Rats Following Acute Myocardial Infarction
复制标题
莫诺苷对急性心肌梗死大鼠的心脏保护作用
DOI：
10.1007/s10753-017-0699-x
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发表时间：
2018-03-01
期刊：
影响因子：
5.1
通讯作者：
Wang, Wen
中科院分区：
文献类型：
--
作者：
Yu, Bangxing;Wang, Wen
摘要本研究旨在探讨莫诺苷对大鼠急性心肌梗死的保护作用。通过结扎冠状动脉前降支（LAD）诱导急性心肌梗死（AMI）[1]。AMI后，莫诺苷分别以45、90和180 mg/kg的剂量灌胃给药24 h。使用市售试剂盒检测AMI大鼠血清中肌酸激酶（CK-MB）、乳酸脱氢酶（LDH）、β-羟基丁酸脱氢酶（β-HBDH）和天冬氨酸转氨酶（AST）活性等生物标志物[2]。AMI后，莫诺苷分别以45、90和180 mg/kg/d的剂量灌胃给药72 h。采用免疫印迹法检测心肌组织核因子κ B（NF-κB）表达。同时用超声心动图测定心功能。观察莫诺苷对AMI大鼠心肌CK-MB、LDH、AST、β-HBDH的影响。Morroniside可降低AMI后72 h大鼠心肌NF-κB的表达。此外，通过给予莫诺苷改善了心脏功能。总的来说，我们的研究结果表明，莫诺苷对大鼠急性心肌梗死具有心脏保护作用。减轻炎症可能有助于莫诺苷的心脏保护作用。
AbstractThe aim of this study is to investigate the cardioprotective effects of morroniside in rats following acute myocardial infarction. An acute myocardial infarction (AMI) was induced by ligating the anterior descending coronary artery (LAD) [1]. Following AMI, morroniside was administered intragastrically for 24 h at doses of 45, 90, and 180 mg/kg, respectively. Biomarkers such as creatine kinase (CK-MB), lactate dehydrogenase (LDH), ɑ-hydroxybutyrate dehydrogenase (ɑ-HBDH), and aspartate aminotransferase (AST) activities in AMI rats in the serum were detected with commercial kits [2]. Following AMI, morroniside was administered intragastrically for 72 h at doses of 45, 90, and 180 mg/kg/d, respectively. The expression of nuclear factor kappa B (NF-κB) in cardiac myocardium was detected by western blotting analysis. Meanwhile, cardiac function was measured by echocardiography. We observed morroniside decreased the levels of CK-MB, LDH, ɑ-HBDH, and AST activities in AMI rats after 24 h. We also found that morroniside reduced the expression of NF-κB in cardiac myocardium at 72 h post AMI rats. Further, cardiac function was improved by administration of morroniside. Collectively, our findings demonstrated that morroniside had cardioprotective effects in rats following acute myocardial infarction. Attenuation of inflammation might contribute to the cardioprotective effects of morroniside.