TANGLED1 mediates microtubule interactions that may promote division plane positioning in maize
TANGLED1 mediates microtubule interactions that may promote division plane positioning in maize
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DOI：
10.1083/jcb.201907184
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发表时间：
2020-08-03
影响因子：
7.8
通讯作者：
Rasmussen, Carolyn G.
中科院分区：
文献类型：
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作者：
Martinez, Pablo;Dixit, Ram;Rasmussen, Carolyn G.
微管细胞骨架是真核细胞有丝分裂的动态结构框架。TANGLED1(TAN1)是一种微管结合蛋白，定位于分裂部位和有丝分裂的微管，在植物的分裂平面定位中起关键作用。在这里，体外实验表明，TAN1直接与微管结合，介导微管拉链或端对端微管相互作用，这取决于它们的接触角。玉米tan‘突变体细胞错误地定位了预测未来分裂位点的前期带(PPB)。然而，基于细胞形状的建模表明，PPB定位缺陷可能是细胞形状异常的结果，而不是由于TAN1的缺失。在末期，从膜质体内生长的微管末端与TAN1共同定位在分裂部位，表明TAN1与微管末端相互作用。综上所述，我们的结果表明，TAN1有助于微管的组织，以确保适当的分工平面方向。
The microtubule cytoskeleton serves as a dynamic structural framework for mitosis in eukaryotic cells. TANGLED1 (TAN1) is a microtubule-binding protein that localizes to the division site and mitotic microtubules and plays a critical role in division plane orientation in plants. Here, in vitro experiments demonstrate that TAN1 directly binds microtubules, mediating microtubule zippering or end-on microtubule interactions, depending on their contact angle. Maize tan' mutant cells improperly position the preprophase band (PPB), which predicts the future division site. However, cell shape-based modeling indicates that PPB positioning defects are likely a consequence of abnormal cell shapes and not due to TAN1 absence. In telophase, colocalization of growing microtubules ends from the phragmoplast with TAN1 at the division site suggests that TAN1 interacts with microtubule tips end-on. Together, our results suggest that TAN1 contributes to microtubule organization to ensure proper division plane orientation.