Cellular Transfer of Magnetic Nanoparticles Via Cell Microvesicles: Impact on Cell Tracking by Magnetic Resonance Imaging
Cellular Transfer of Magnetic Nanoparticles Via Cell Microvesicles: Impact on Cell Tracking by Magnetic Resonance Imaging
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DOI：
10.1007/s11095-012-0680-1
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发表时间：
2012-05-01
影响因子：
3.7
通讯作者：
Gazeau, Florence
中科院分区：
文献类型：
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作者：
Silva, Amanda K. Andriola;Wilhelm, Claire;Gazeau, Florence
磁性纳米颗粒细胞标记可用于通过磁共振成像监测体内移植细胞的命运。然而，最初内化在施用细胞中的纳米颗粒最终可能会进入宿主生物体的其他细胞中。我们研究了磁性纳米颗粒通过细胞应激下释放的细胞微泡向不同类型受体细胞进行细胞间交叉转移的机制。三种细胞类型（间充质干细胞、内皮细胞和巨噬细胞）用 8 纳米氧化铁纳米颗粒标记。然后细胞经历饥饿应激，在此期间它们产生微泡，随后将其转移到未标记的受体细胞中。对供体细胞的磁泳迁移率的分析表明，在细胞应激下磁负荷部分丧失。应激细胞脱落的微泡参与了磁性标记的释放。此外，此类微泡被幼稚细胞摄取，导致纳米颗粒在细胞中重新分布。受体细胞的铁负荷允许通过 MRI 进行检测。在压力下释放的细胞微泡可能会在整个生物体中传播，并被宿主细胞摄取。转移的货物可能足以让 MRI 检测到这些二次标记的细胞，从而导致对移植细胞有效性的误解。
Cell labeling with magnetic nanoparticles can be used to monitor the fate of transplanted cells in vivo by magnetic resonance imaging. However, nanoparticles initially internalized in administered cells might end up in other cells of the host organism. We investigated a mechanism of intercellular cross-transfer of magnetic nanoparticles to different types of recipient cells via cell microvesicles released under cellular stress.Three cell types (mesenchymal stem cells, endothelial cells and macrophages) were labeled with 8-nm iron oxide nanoparticles. Then cells underwent starvation stress, during which they produced microvesicles that were subsequently transferred to unlabeled recipient cells.The analysis of the magnetophoretic mobility of donor cells indicated that magnetic load was partially lost under cell stress. Microvesicles shed by stressed cells participated in the release of magnetic label. Moreover, such microvesicles were uptaken by na < ve cells, resulting in cellular redistribution of nanoparticles. Iron load of recipient cells allowed their detection by MRI.Cell microvesicles released under stress may be disseminated throughout the organism, where they can be uptaken by host cells. The transferred cargo may be sufficient to allow MRI detection of these secondarily labeled cells, leading to misinterpretations of the effectiveness of transplanted cells.