Zona pellucida family genes in Chinese pond turtle: identification, expression profiles, and role in the spermatozoa acrosome reaction†
Zona pellucida family genes in Chinese pond turtle: identification, expression profiles, and role in the spermatozoa acrosome reaction†
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DOI：
10.1093/biolre/ioad049
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发表时间：
2023-05-04
影响因子：
3.6
通讯作者：
Nie，Liuwang
中科院分区：
文献类型：
--
作者：
Dong，Jinxiu;Pei，Kejiao;Nie，Liuwang
透明带（ZP）是包围所有脊椎动物卵的细胞外基质，它参与受精和物种特异性识别。人们对哺乳动物、鸟类、两栖动物和鱼类的ZP蛋白进行了大量深入的研究，但对ZP家族基因及其在爬行动物受精过程中的作用的系统研究迄今尚未见报道。本研究基于海龟全基因组序列数据，鉴定了6个海龟ZP （Tu-ZP）基因亚家族（Tu-ZP1、Tu-ZP2、Tu-ZP3、Tu-ZP4、Tu-ZPD和Tu-ZPAX）。我们发现tu - zp4具有较大的片段重复，分布在三条染色体上，我们也在其他Tu-ZP基因中检测到基因重复。为了评估Tu-ZP蛋白在精卵结合中的作用，我们评估了这些Tu-ZP蛋白在m中的表达模式及其诱导精子顶体反应的能力。reevesii。综上所述，本研究首次报道了Tu-ZP2基因的重复存在，以及Tu-ZP2、Tu-ZP3和Tu-ZPD可以诱导爬行动物精子发生的顶体胞吐。
The zona pellucida (ZP) is an extracellular matrix that surrounds all vertebrate eggs, and it is involved in fertilization and species-specific recognition. Numerous in-depth studies of the ZP proteins of mammals, birds, amphibians, and fishes have been conducted, but systematic investigation of the ZP family genes and their role during fertilization in reptiles has not been reported to date. In this study, we identified six turtle ZP (Tu-ZP) gene subfamilies (Tu-ZP1, Tu-ZP2, Tu-ZP3, Tu-ZP4, Tu-ZPD, and Tu-ZPAX) based on whole genome sequence data fromMauremys reevesii. We found thatTu-ZP4had large segmental duplication and was distributed on three chromosomes, and we also detected gene duplication in the other Tu-ZP genes. To evaluate the role of Tu-ZP proteins in sperm–egg binding, we assessed the expression pattern of these Tu-ZP proteins and their ability to induce the spermatozoa acrosome reaction inM. reevesii. In conclusion, this is the first report of the existence of gene duplication ofTu-ZPgenes and that Tu-ZP2, Tu-ZP3, and Tu-ZPD can induce acrosome exocytosis of spermatogenesis in the reptile.