Epiphytic bacterial communities on two common submerged macrophytes in Taihu Lake: diversity and host-specificity
Epiphytic bacterial communities on two common submerged macrophytes in Taihu Lake: diversity and host-specificity
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DOI：
10.1007/s00343-012-1084-0
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发表时间：
2012-03-01
影响因子：
--
通讯作者：
Wu Qinglong
中科院分区：
文献类型：
--
作者：
He Dan;Ren Lijuan;Wu Qinglong
陆生和水生植物的叶子是各种细菌物种的家园。然而，附生细菌在沉水植物寄主上的多样性和变异性仍然知之甚少。针对细菌16S rRNA基因，采用末端限制性片段长度多态分析和克隆文库分析的方法，对江苏太湖常见沉水植物--苦草和黑藻附生细菌的多样性和组成进行了研究。结果表明：(1)两种水藻的文库中含有广泛的细菌系统发育分布，两个文库的序列可划分为93个OTU(97%的相似度)；(2)包括伯克霍尔德雷氏菌在内的Beta-Protecteria是两种植物中最丰富的细菌类群。在纳氏弧菌和轮纹夜蛾上，蓝藻和伽马蛋白细菌分别是第二大类群。两个克隆文库都包含一些与甲烷氧化菌和固氮菌相关的序列；(3)T-RFLP图谱的聚类分析显示，两个植物种群对应着两个不同的簇。T-RFLP数据的ANOSIM和两个克隆文库的LibShuff分析都表明两种植物附生细菌群落存在显著差异。因此，沉水植物附生细菌群落具有多样性和寄主特异性，这可能有助于从总体上理解沉水植物的生态功能。
Leaves of terrestrial and aquatic plants are home to a wide diversity of bacterial species. However, the diversity and variability of epiphytic bacteria on their submerged plant hosts remains poorly understood. We investigated the diversity and composition of epiphytic bacteria from two common submerged macrophytes: Vallisneria natans and Hydrilla verticillata in Taihu Lake, Jiangsu, China, using methods of terminal restriction fragment length polymorphisms (T-RFLP) and clone library analyses targeted at bacterial 16S rRNA genes. The results show that: (1) the libraries of the two waterweeds contain wide phylogenetic distribution of bacteria, and that the sequences of the two libraries can be separated into 93 OTUs (at 97% similar value); (2) Betaproteobacteria, including Burkholderiales, was the most abundant bacterial group on both plants. Cyanobacteria and Gammaproteobacteria were the second largest groups on V. natans and H. verticillata, respectively. Both clone libraries included some sequences related to those of methanotrophs and nitrogen-fixing bacteria; (3) Cluster analysis of the T-RFLP profiles showed two distinct clusters corresponding to the two plant populations. Both ANOSIM of the T-RFLP data and Libshuff analysis of the two clone libraries indicated a significant difference in epiphytic bacterial communities between the two plants. Therefore, the epiphytic bacterial communities on submerged macrophytes appear to be diverse and host-specific, which may aid in understanding the ecological functions of submerged macrophytes in general.