Spencerozyma acididurans sp. nov., an acid-tolerant basidiomycetous yeast species isolated from acid mine drainage
Spencerozyma acididurans sp. nov., an acid-tolerant basidiomycetous yeast species isolated from acid mine drainage
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Spencerozyma Acididurans sp.
DOI：
10.1099/ijsem.0.003565
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发表时间：
2019
影响因子：
2.8
通讯作者：
Li-Nan Huang
中科院分区：
文献类型：
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作者：
Bei-Cheng Yang;Nan Chen;Bin Liao;Yong Huang;Li-Nan Huang
菌株SYSU-17是从广东省凡口铅锌矿尾矿库酸性矿山废水中分离得到的一种耐酸酵母菌，该酵母菌可在pH 2.0条件下生长。基于内部转录间隔区（ITS）和大亚基核糖体RNA （LSU rRNA）基因D1/D2结构域的系统发育分析表明，菌株SYSU-17属于spencerozyma属（微孢菌纲，puccininiomycotina亚门）新种。在LSU rRNA基因的D1/D2结构域上，它与最近的亲缘种Spencerozyma croceaCBS 2029T型菌株的序列差异为0.7% （594 bp的3个缺口和1个核苷酸替换），在ITS区域的序列差异为7.6% （714 bp的32个缺口和22个核苷酸替换）。与s。新酵母Crocea不能吸收半乳糖、d-核糖、木糖醇、琥珀酸盐、d-木糖、乙醇、硝酸盐和亚硝酸盐。其名称为酸性酵素。11月被提出，SYSU-17被指定为全模。
Strain SYSU-17, representing a novel acid-tolerant yeast species which can grow at pH 2.0 weakly, was isolated from acid mine drainage collected in a tailing impoundment of the Fankou Lead/Zinc Mine, Guangdong Province, PR China. Phylogenetic analysis of strain SYSU-17 based on the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit ribosomal RNA (LSU rRNA) gene suggested that strain SYSU-17 was a novel species belonging to the genusSpencerozyma(class Microbotryomycetes, subphylum Pucciniomycotina). It differed from the type strain of the closest related species,Spencerozyma croceaCBS 2029T, by 0.7 % sequence divergence (three gaps and one nucleotide substitution out of 594 bp) in the D1/D2 domains of the LSU rRNA gene and 7.6 % sequence divergence (32 gaps and 22 nucleotide substitutions out of 714 bp) in the ITS region. In contrast to the physiological properties ofS. crocea, the novel yeast species was unable to assimilate galactose,d-ribose, xylitol, succinate,d-xylose, ethanol, nitrate and nitrite. The nameSpencerozyma acididuranssp. nov. is proposed and SYSU-17 is designated as the holotype.