ELECTRON-TRANSFER PROPERTIES OF MELANIN
ELECTRON-TRANSFER PROPERTIES OF MELANIN
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DOI：
10.1016/0003-9861(76)90304-0
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发表时间：
1976-01-01
影响因子：
3.9
通讯作者：
MENON, IA
中科院分区：
文献类型：
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作者：
GAN, EV;HABERMAN, HF;MENON, IA
由蘑菇酪氨酸酶和3,4-二羟基苯丙氨酸合成的黑色素已被证明可以氧化NADH和NADPH，还原铁氰化物，氧化形式的细胞色素和二氯酚吲哚酚，并催化NADH和铁氰化物的偶联氧化。动力学研究包括测定不同浓度底物的初始速度和对nadh -铁氰化物-黑色素反应的产物抑制测量。结果与乒乓机制一致，即在黑色素与第二底物反应之前形成一个产物，在反应过程中涉及黑色素的可逆氧化和还原。由此可以得出结论，黑色素在几种还原-氧化系统中可以作为电子转移剂。
Melanin synthesized from mushroom tyrosinase and 3,4-dihydroxyphenylalanine has been shown to oxidize NADH and NADPH, reduce ferricyanide, oxidized forms of cytochromecand dichlorophenolindophenol, and catalyze the coupled oxidation of NADH and reduction of ferricyanide. Kinetic studies involving the determination of initial velocity at various concentrations of substrates and product inhibition measurements have been carried out on the NADH-ferricyanide-melanin reaction. The results are consistent with a ping-pong mechanism in which one product is formed prior to the reaction of melanin with the second substrate involving the reversible oxidation and reduction of melanin during the reaction. It may be concluded that melanin is capable of acting as an electron transfer agent in several reduction-oxidation systems.