δ-Tubulin is a component of intercellular bridges and both the early and mature perinuclear rings during spermatogenesis
δ-Tubulin is a component of intercellular bridges and both the early and mature perinuclear rings during spermatogenesis
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DOI：
10.1016/j.ydbio.2004.01.026
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发表时间：
2004-05-01
影响因子：
2.7
通讯作者：
Todokoro, K
中科院分区：
文献类型：
--
作者：
Kato, A;Nagata, Y;Todokoro, K
哺乳动物精子发生涉及导致成熟精子发育的剧烈形态变化。精子的发育包括顶体和鞭毛的形成、核-顶体向细胞表面的移位、核的浓缩和伸长。此外，生精细胞后代分化为通过细胞间桥相互连接的单元群。关于精子细胞的结构组成和雄配子核形成的机制还知之甚少。我们确定了两种亚型的δ-微管蛋白，并发现长亚型主要在睾丸中表达，而短亚型在所有检查的组织中表达。我们还发现，δ-微管蛋白形成细胞间桥连接姐妹生精细胞。此外，δ-微管蛋白是一个组成部分的核周环的曼切特，其作用于易位和延长的核。圆形精子细胞的细胞表面还存在明显区别于细胞间桥的小环，在精子发生的后期，小环可能发育成核周环。这些结果表明，δ-微管蛋白是两种类型的环，细胞间桥和核周环的组成部分，这可能是参与精子细胞的形态变化，以成熟精子。(C)2004年爱思唯尔公司All rights reserved.
Mammalian spermatogenesis involves drastic morphological changes leading to the development of the mature sperm. Sperm development includes formation of the acrosome and flagellum, translocation of nucleus-acrosome to the cell surface, and condensation and elongation of the nucleus. In addition, spermatogenic cell progenies differentiate as cohorts of units interconnected by intercellular bridges. Little is known about the structural components involved in the establishment of conjoined spermatogenic cells and the mechanism of nuclear shaping of the male gamete. We identified two isoforms of delta-tubulin and found that the long isoform is predominantly expressed in testis, while the short isoform is expressed in all tissues examined. We also found that delta-tubulin forms intercellular bridges conjoining sister spermatogenic cells. In addition, delta-tubulin is a component of the perinuclear ring of the manchette, which acts on translocation and elongation of the nucleus. Furthermore, small rings clearly distinct from the intercellular bridges, which might mature to perinuclear ring of the manchette in later stages of spermatogenesis, were detected on the cell surface of round spermatids. These results suggest that delta-tubulin is a component of two types of ring, the intercellular bridges and the perinuclear rings, which may be involved in morphological changes of spermatid to mature sperm. (C) 2004 Elsevier Inc. All rights reserved.