Photoinduced crosslinking of double-helical DNA by psoralen covalently linked to a triple helix-forming oligonucleotide under near-physiological conditions.
Photoinduced crosslinking of double-helical DNA by psoralen covalently linked to a triple helix-forming oligonucleotide under near-physiological conditions.
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在接近生理条件下，补骨脂素与形成三螺旋的寡核苷酸共价连接，导致双螺旋 DNA 发生光诱导交联。
DOI：
10.1080/15257770701508554
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Li H
通过将C和T碱基类似物3-甲基-2-氨基吡啶-2 ′-脱氧核苷和5-（3-氨基丙-2-炔基）-′-脱氧尿苷引入到peptien缀合的三链体形成寡核苷酸中，在接近生理条件下产生了稳定的三链体。用365 nm的紫外光照射后，通过紫外熔解分析和荧光测量观察到TFO与双螺旋DNA的光诱导交联。
Stable triplexes have been generated under near-physiological conditions by the introduction of the C and T base analogues 3-methyl-2-aminopyridine-2 ′-deoxyriboside and 5-(3-aminoprop-2-ynyl)-′-deoxyuridine into psoralen-conjugated triplex-forming oligonucleotides. After irradiation with UV light at 365 nm, photo-induced cross-linking of the TFO to double-helical DNA was observed by UV-melting analysis and fluorescence measurements.