Assessment of the stability of catechin-enriched extracts obtained from Arbutus unedo L. fruits: Kinetic mathematical modeling of pH and temperature properties on powder and solution systems
Assessment of the stability of catechin-enriched extracts obtained from Arbutus unedo L. fruits: Kinetic mathematical modeling of pH and temperature properties on powder and solution systems
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DOI：
10.1016/j.indcrop.2017.02.002
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发表时间：
2017-05
影响因子：
5.9
通讯作者：
B. Albuquerque;M. Prieto;L. Barros;I. Ferreira
中科院分区：
文献类型：
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作者：
B. Albuquerque;M. Prieto;L. Barros;I. Ferreira
杨梅属植物(草莓)水果可以被认为是黄烷-3-醇的替代来源，特别是儿茶素及其衍生物。这些化合物在食品(特别是作为食品添加剂)、制药和化妆品工业中有着广泛的应用。因此，可以得到富含儿茶素的提取物(60%的黄烷醇和22%的儿茶素)。考虑到主要影响变量(时间、温度和pH)：i)提取物在获取和储存过程中的稳定性研究(粉末体系)；以及ii)提取物在模拟食品环境中的稳定性研究(水溶液体系)。测定的反应是黄烷醇和儿茶素的含量，用高效液相色谱与二极管阵列检测器(HPLCDAD)联用测定，并用亲水性分析评价提取物的抗氧化活性。用机理方程和唯象方程描述了黄烷醇(包括儿茶素)作为粉末提取物在一个月内的稳定性，其最佳条件是pH=5.4T_(−)=20°C，而其在水溶液中的稳定性在pH=4和T=30°C条件下24小时内保持不变。作为黄烷-3-醇的替代来源的茄果；以及ii)在特定温度和pH条件下的货架期计算和儿茶素损失预测。最后，所得结果与以前关于儿茶素在粉剂和溶液系统中稳定性研究的报道有一定的一致性，但提供了一种新的替代来源：文冠果。
Arbutus unedoL. (strawberry-tree) fruits could be considered an alternative source of flavan-3-ols, in particular catechin and its derivatives. These compounds are known for a variety of applications in food (especially as food additives), pharmaceutical and cosmetic industry. Therefore, catechin-enriched extracts (60% flavan-3-ols and 22% of catechin) were obtained fromA. unedofruits and further submitted to physical and chemical stability studies, considering the main affecting variables (time, temperature and pH): i) a stability study of the extracts during the obtaining and storage procedures (powder system); and ii) a stability study of the extracts in simulated food environment (aqueous solution system). The measured responses were the flavan-3-ols and catechin contents, determined by High performance liquid chromatography couple to a diode array detector (HPLC-DAD), and the antioxidant activity of the extracts evaluated by hydrophilic assays. Mechanistic and phenomenological equations were used to describe the responses, and the optimal conditions for flavan-3-ols (including catechin) stability as powder extract during a month were pH = 5.4 and T = −20 °C; while its stability in aqueous solution remained during the 24 h of application at pH <4 and T < 30 °C. These results provide useful information for: i) potential industrial use ofA. unedofruits as alternative sources of flavan-3-ols; and ii) shelf-life calculations and catechin loss predictions at specific conditions of temperature and pH. Finally, the results obtained showed a certain agreement with previous reports of catechin stability studies in powder and solution systems, but providing a new alternative source:A. unedofruits.