PIGMENT MIGRATION AND ADAPTATION IN EYE OF SQUID, LOLIGO-PEALEI
PIGMENT MIGRATION AND ADAPTATION IN EYE OF SQUID, LOLIGO-PEALEI
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DOI：
10.1085/jgp.63.1.22
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发表时间：
1974-01-01
影响因子：
3.8
通讯作者：
PEARLMAN, AL
中科院分区：
文献类型：
--
作者：
DAW, NW;PEARLMAN, AL
研究了筛选色素在完整鱿鱼视网膜中的迁移。色素迁移的作用谱与视紫红质的作用谱相对应，而不是与筛选色素的吸收谱相对应。一个固定的色素迁移标准所需的量子总数是相同的，当量子在从6秒到1小时或更长时间的任何时间段内传递时。当少于3-10%的视紫红质异构化时，筛选色素以5-15分钟的时间迁移到受体的尖端，并在相同的时间内再次返回。当异构化超过10%时，向外迁移需要5-15分钟，但筛选色素不向内迁移，即使在黑暗中待了几个小时。间接证据表明，筛选色素的波段，当它到达受体的尖端时，大约相当于0.6 log单位的过滤器。测定了视神经反应的光谱灵敏度，发现其比鱿鱼视紫红质的体外吸收光谱更宽；宽度可以通过自筛选来解释，假设在500 nm处紫红质密度为0.6 log单位。
The migration of the screening pigment was investigated in the retina of the intact squid. The action spectrum of pigment migration corresponds to the action spectrum of the visual pigment, rhodopsin, rather than to the absorption spectrum of the screening pigment. The total number of quanta required for a fixed criterion of pigment migration is the same, when the quanta are delivered over any period of time from 6 s to an hour or more. When less than 3-10% of the rhodopsin is isomerized, the screening pigment migrates out to the tips of the receptors with a time-course of 5-15 min, and back again over the same period of time. When rather more than 10% is isomerized, the outward migration takes 5-15 min, but the screening pigment does not migrate inwards, even after several hours in the dark. Indirect evidence suggests that the band of screening pigment, when it reaches the tips of the receptors, is approximately equivalent to a filter of 0.6 log units. The spectral sensitivity of the optic nerve response was measured, and was found to be broader than the absorption spectrum of squid rhodopsin in vitro; the broadness could be explained by self-screening, assuming a density of rhodopsin of 0.6 log units at 500 nm.