Beneficial Effects of the Genus Aloe on Wound Healing, Cell Proliferation, and Differentiation of Epidermal Keratinocytes.
Beneficial Effects of the Genus Aloe on Wound Healing, Cell Proliferation, and Differentiation of Epidermal Keratinocytes.
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DOI：
10.1371/journal.pone.0164799
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Hayakawa T
中科院分区：
文献类型：
--
作者：
Moriyama M;Moriyama H;Uda J;Kubo H;Nakajima Y;Goto A;Akaki J;Yoshida I;Matsuoka N;Hayakawa T
芦荟一直被用作民间药物，因为它有几个重要的治疗作用。这些药物包括伤口和烧伤愈合，芦荟现在被用于各种商业可用局部药物，用于伤口愈合和皮肤护理。然而，它对表皮角质形成细胞的影响在很大程度上还不清楚。我们的数据表明，芦荟凝胶(AVG)和开普芦荟提取物(CAE)都显著促进了人原代表皮角质形成细胞(HPEK)和相当于人皮肤模型的伤口愈合。此外，流式细胞仪分析显示，β1-、α6-、β4-整合素和E-钙粘素在细胞表面的表达增加。这些增加可能有助于细胞迁移和伤口愈合。芦荟处理还导致细胞周期进程的显著变化和细胞数量的增加。芦荟提高HPEK分化标志物的基因表达，提示AVG和CAE在改善角质形成细胞功能中起作用。此外，在含有芦荟的培养液中培养的人皮肤表皮当量物比对照皮肤当量物厚，这表明芦荟对促进表皮发育有效。基于这些结果，AVG和CAE在伤口愈合和治疗粗糙皮肤方面都有好处。
Aloe has been used as a folk medicine because it has several important therapeutic properties. These include wound and burn healing, and Aloe is now used in a variety of commercially available topical medications for wound healing and skin care. However, its effects on epidermal keratinocytes remain largely unclear. Our data indicated that both Aloe vera gel (AVG) and Cape aloe extract (CAE) significantly improved wound healing in human primary epidermal keratinocytes (HPEKs) and a human skin equivalent model. In addition, flow cytometry analysis revealed that cell surface expressions of β1-, α6-, β4-integrin, and E-cadherin increased in HPEKs treated with AVG and CAE. These increases may contribute to cell migration and wound healing. Treatment with Aloe also resulted in significant changes in cell-cycle progression and in increases in cell number. Aloe increased gene expression of differentiation markers in HPEKs, suggesting roles for AVG and CAE in the improvement of keratinocyte function. Furthermore, human skin epidermal equivalents developed from HPEKs with medium containing Aloe were thicker than control equivalents, indicating the effectiveness of Aloe on enhancing epidermal development. Based on these results, both AVG and CAE have benefits in wound healing and in treatment of rough skin.
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影响因子：
3.1
作者：
O'Brien, C.;Van Wyk, B. -E.;Van Heerden, F. R.
通讯作者：
Van Heerden, F. R.
Acemannan 通过 AKT/mTOR 信号通路加速细胞增殖和皮肤伤口愈合
影响因子：
4.6
作者：
Xing, Wei;Guo, Wei;Xu, Xiang
通讯作者：
Xu, Xiang
影响因子：
4.8
作者：
Hwang, Soonyean;Zimmerman, Noah P.;Dwinell, Michael B.
通讯作者：
Dwinell, Michael B.
DOI：
10.1083/jcb.200509084
发表时间：
2006-05-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Moriyama M;Osawa M;Mak SS;Ohtsuka T;Yamamoto N;Han H;Delmas V;Kageyama R;Beermann F;Larue L;Nishikawa S
通讯作者：
Nishikawa S
DOI：
10.7547/87507315-84-2-77
发表时间：
1994-02-01
影响因子：
0.7
作者：
DAVIS, RH;DIDONATO, JJ;HAAS, RC
通讯作者：
HAAS, RC