Variation in fatty acid composition of Arthrospira (Spirulina) strains
Variation in fatty acid composition of Arthrospira (Spirulina) strains
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DOI：
10.1007/s10811-005-7213-9
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发表时间：
2005-03-01
影响因子：
3.3
通讯作者：
Whitton, BA
中科院分区：
文献类型：
--
作者：
Mühling, M;Belay, A;Whitton, BA
对35株节旋藻的脂肪酸组成进行了研究，主要集中在脂肪酸含量最丰富的两种多不饱和C-18酸、亚油酸和γ-亚麻酸以及棕榈酸。在30℃低光照条件下生长时，这三种酸占总脂肪酸的88-92%。这两种多不饱和酸的组成有相当大的差异。根据菌株的不同，亚油酸占总脂肪酸的13.1-31.5%，伽马-亚麻酸占总脂肪酸的12.9-29.4%。相比之下，棕榈酸的含量范围较窄：占总脂肪酸的42.3-47.6%。在特定条件下对几个菌株进行重复实验，在任何特定环境下都会得到非常相似的结果，这表明脂肪酸组成可以作为区分菌株的辅助手段。另外5个菌株也进行了检测，这些菌株显然来自主要研究中35个菌株中的3个，但来自不同的培养集合。四个菌株的结果是相似的，不考虑培养来源，但一个菌株出现了显著的差异，特别是在多不饱和C-18脂肪酸部分。这些差异与文化的时代无关。此外，在四个菌株的重复继代培养过程中产生的直线型；每个菌株都显示出与相同菌株的螺旋形态相似的脂肪酸组成。温度从30℃下降到20℃，辐照度(30℃)从10 mU·m~(-2)增加到70亩·m~(-2)·S(~(-1))，以及向暗异养转变，都有利于多不饱和C-18脂肪酸的增加。最高的。-在30摄氏度的黑暗中异养生长在葡萄糖上的三个菌株在任何条件下的亚麻酸含量都被发现。对六个螺旋藻菌株的比较研究证实了之前的一项研究表明没有亚麻酸。-亚麻酸在所有螺旋藻菌株中，从而允许这两个属的分离。
A study of the fatty acid composition was made for 35 Arthrospira strains, concentrating on the most abundant fatty acids, the two polyunsaturated C-18 acids, linoleic and gamma-linolenic acid, and palmitic acid. When grown at 30 degrees C and low irradiance (10 mu mol photon m(-2) s(-1)), these three acids together formed 88 - 92% of total fatty acids. There were considerable differences in the composition of the two polyunsaturated acids. Depending on the strain, linoleic acid formed 13.1 - 31.5% and gamma-linolenic acid formed 12.9 - 29.4% total fatty acids. In contrast, the range for palmitic acid was narrow: 42.3 - 47.6% of total fatty acids. Repeat experiments on several strains under defined conditions led to closely similar results for any particular environment, suggesting that fatty acid composition can be used as an aid in differentiating between strains. Five additional strains, which had apparently originated from the same original stock cultures as 3 of the 35 in the main study, but from different culture collections, were also assayed. With four strains the results were similar, irrespective of culture source, but with one strain marked differences occurred, especially in the polyunsaturated C-18 fatty acid fraction. These differences were independent of the age of the culture. In addition, straight morphotypes derived during repeat subcultures of four strains; each showed a similar fatty acid composition to that of the helical morphotypes of the same strains. A decrease in temperature from 30 to 20 degrees C, an increase in irradiance ( at 30 degrees C) from 10 to 70 mu mol photon m(-2) s(-1) and transfer to dark heterotrophy all favoured an increase in polyunsaturated C-18 fatty acids. The highest. - linolenic acid content of any conditions was found for three strains grown heterotrophically on glucose in the dark at 30 degrees C. A comparative study of six strains of Spirulina confirmed a previous study showing the absence of. - linolenic acid in all Spirulina strains, thus permitting the separation of these two genera.