Observing a molecular knife at work
Observing a molecular knife at work
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DOI：
10.1021/ja057029t
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发表时间：
2006-03-01
影响因子：
15
通讯作者：
Chen, Z
中科院分区：
文献类型：
--
作者：
Chen, XY;Tang, HZ;Chen, Z
基于天然抗菌肽的共同结构基序，采用和频振动光谱（SFG）研究了单底物支撑的脂双层膜与两亲性抗生素化合物1之间的分子相互作用. SFG的界面灵敏度允许同时在双层的两个小叶和1的取向中实时原位监测有序变化。发现1的临界浓度约为0.8 μ g/mL，高于该浓度，双层的内小叶被显著扰动。该浓度很好地对应于从细菌实验获得的最小抑制浓度1。1在双层中的取向被示出为垂直于双层表面，与模拟结果一致。SFG可以发展成为研究细胞膜和膜活性分子相互作用的一种非常有用的技术。
Sum frequency generation (SFG) vibrational spectroscopy has been employed to study the molecular interactions between a single substrate supported lipid bilayer and an amphiphilic antibiotic compound 1, with a design based on the common structural motif of natural antimicrobial peptides. The interfacial sensitivity of SFG allows real-time in situ monitoring of ordering changes in both leaflets of the bilayer and orientation of 1 simultaneously. A critical concentration of about 0.8 mu g/mL of 1 is found, above which the inner leaflet of the bilayer is significantly perturbed. This concentration corresponds well to the minimum inhibition concentration of 1 that is obtained from bacterial experiments. Orientation of 1 in the bilayer is shown to be perpendicular to the bilayer surface, in agreement with simulation results. SFG can be developed into a very informative technique for studying the cell membrane and the interactions of membrane-active molecules.