EST analysis of male accessory glands from Heliconius butterflies with divergent mating systems
EST analysis of male accessory glands from Heliconius butterflies with divergent mating systems
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DOI：
10.1186/1471-2164-9-592
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发表时间：
2008-12-08
期刊：
影响因子：
4.4
通讯作者：
Harrison, Richard G.
中科院分区：
文献类型：
--
作者：
Walters, James R.;Harrison, Richard G.
工作背景：Heliconius蝴蝶具有显着的多样性的表型，生理和行为，长期以来一直区分本属作为生态和进化研究的重点类群。最近，Heliconius也成为使用基因组方法研究生物多样性的原因和后果的模型系统。Heliconius多样性的一个值得注意的方面是交配系统的二分法，这为研究性选择和生殖蛋白进化之间的关系提供了一个不寻常的机会。作为这项研究的第一步，我们报道了从雄性附腺中提取表达序列标签（EST）并进行分析。erato和H.结果：成功测序了933条EST序列，共371个单基因，其中371个为单基因。erato和1033个EST聚为340个单基因。美波美两个物种的结果非常相似。大约三分之一的unigenes显示与GenBank的非冗余数据库中的序列没有显著的BLAST相似性（E值< 10（-5）），表明大比例的新基因在Heliconius雄性附腺中表达。在这两个物种中，只有三分之一的附属腺unigenes也被发现在翅组织中表达的基因。约25%的unigenes从两个物种编码分泌蛋白。这包括三组高度丰富的编码重复蛋白的单基因，这些重复蛋白被认为是候选的精液蛋白;在H.结论：这些EST的收集将作为未来的鉴定和进化分析的雄性生殖蛋白的Heliconius蝴蝶的基础。这些数据也代表了一个显着的进步，在快速增长的收集基因组资源可在Heliconius蝴蝶。因此，他们大大提高了这个分类群作为一个模型系统，调查生态，表型和基因组多样性的问题。
Background: Heliconius butterflies possess a remarkable diversity of phenotypes, physiologies, and behaviors that has long distinguished this genus as a focal taxon in ecological and evolutionary research. Recently Heliconius has also emerged as a model system for using genomic methods to investigate the causes and consequences of biological diversity. One notable aspect of Heliconius diversity is a dichotomy in mating systems which provides an unusual opportunity to investigate the relationship between sexual selection and the evolution of reproductive proteins. As a first step in pursuing this research, we report the generation and analysis of expressed sequence tags (ESTs) from the male accessory gland of H. erato and H. melpomene, species representative of the two mating systems present in the genus Heliconius.Results: We successfully sequenced 933 ESTs clustering into 371 unigenes from H. erato and 1033 ESTs clustering into 340 unigenes from H. melpomene. Results from the two species were very similar. Approximately a third of the unigenes showed no significant BLAST similarity (E-value < 10(-5)) to sequences in GenBank's non-redundant databases, indicating that a large proportion of novel genes are expressed in Heliconius male accessory glands. In both species only a third of accessory gland unigenes were also found among genes expressed in wing tissue. About 25% of unigenes from both species encoded secreted proteins. This includes three groups of highly abundant unigenes encoding repetitive proteins considered to be candidate seminal fluid proteins; proteins encoded by one of these groups were detected in H. erato spermatophores.Conclusion: This collection of ESTs will serve as the foundation for the future identification and evolutionary analysis of male reproductive proteins in Heliconius butterflies. These data also represent a significant advance in the rapidly growing collection of genomic resources available in Heliconius butterflies. As such, they substantially enhance this taxon as a model system for investigating questions of ecological, phenotypic, and genomic diversity.