EFFECT OF DEXAMETHASONE ON THE DEVELOPMENT OF RAT INTESTINAL BRUSH-BORDER ENZYMES IN ORGAN-CULTURE
EFFECT OF DEXAMETHASONE ON THE DEVELOPMENT OF RAT INTESTINAL BRUSH-BORDER ENZYMES IN ORGAN-CULTURE
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DOI：
10.1016/0012-1606(80)90049-4
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发表时间：
1980-01-01
影响因子：
2.7
通讯作者：
HAFFEN, K
中科院分区：
文献类型：
--
作者：
KEDINGER, M;SIMON, PM;HAFFEN, K
The effect of dexamethasone on the evolution pattern of brush border enzymes was examined in the rat jejunum cultured in vitro at different postnatal stages (4-21 days). Enzymic activities were analyzed in purified brush border membranes isolated from noncultured intestine and from explants cultured for 24 and 48 h. Dexamethasone exhibited 2 types of effects on the cultured intestinal tissue: a nonspecific but protective effect against the drastic drop of all enzyme activities and against a loss of villus cells observed in control cultures, and a direct and specific effect on precocious induction of sucrase and on stimulation of maltase activity. The SDS[sodium dodecyl sulfate]-polyacrylamide gel patterns of brush border membrane proteins showed that in the 6 day old intestine, appearance of sucrase and stimulation of maltase activities elicited by dexamethasone were accompanied by a simultaneous appearance or enhancement of the corresponding protein bands. The radioactivity peaks on gels due to the incorporation of 14C-valine and of 14C-fucose indicated that dexamethasone induces the synthesis of new proteins or at least the glycosylation of preexisting proteins which may lead to the formation of active maltase and sucrase molecules.
