Diagnostic Algorithm in Hirschsprung's Disease: Focus on Immunohistochemistry Markers
Diagnostic Algorithm in Hirschsprung's Disease: Focus on Immunohistochemistry Markers
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DOI：
10.21873/invivo.11913
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发表时间：
2020-05-01
期刊：
影响因子：
2.3
通讯作者：
Marszalek, Andrzej
中科院分区：
文献类型：
--
作者：
Galazka, Przemyslaw;Szylberg, Lukasz;Marszalek, Andrzej
背景/目的：巨结肠病（HD）是由于肠远端神经节细胞的先天性缺失（神经节病）引起的。直肠活检被认为对其诊断很重要。本研究的目的是应用免疫组织化学染色，使用最小的抗体集，并开发一种算法，将有助于HD的诊断。患者和方法：对HD患者（n=26）或其他肠道疾病患者（n=34）进行直肠或结肠活检。对MAP1b、peripherin、S-100、calretinin、NSE、bcl-2和CD56蛋白进行免疫组化染色。结果：CD56、S-100、外周蛋白和calretinin染色有利于神经节细胞的鉴别。同时使用CD56和S-100抗体可获得最高的神经节细胞染色率（94%）。结论：我们提出了一种应用CD56和S-100抗体的实用诊断算法，可用于临床疑似先天性巨结肠患儿。
Background/Aim: Hirschsprung disease (HD) is caused by the congenital absence of ganglion cells in the distal bowel (aganglionosis). Rectal biopsy is considered important for its diagnosis. The aim of this study was to apply immunohistochemical staining using a minimal set of antibodies and develop an algorithm that will assist in the diagnosis of HD. Patients and Methods: Rectal or colonic biopsies were performed in patients with HD (n=26) or patients treated for other bowel diseases (n=34). Immunohistochemical staining was performed for MAP1b, peripherin, S-100, calretinin, NSE, bcl-2 and CD56 proteins. Results: Staining for CD56, S-100, peripherin and calretinin facilitated the identification of ganglion cells. The use of CD56 and S-100 antibodies together resulted in the highest rate of ganglion cell staining intensity (94%). Conclusion: We propose a practical diagnostic algorithm with the application of CD56 and S-100 antibodies that can be used in clinical practice in children suspected of Hirschsprung's disease.