Presence of robust circadian clock oscillation under constitutive over‐expression of mCry1 in rat‐1 fibroblasts
Presence of robust circadian clock oscillation under constitutive over‐expression of mCry1 in rat‐1 fibroblasts
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DOI：
10.1016/j.febslet.2007.07.053
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发表时间：
2007-08
期刊：
影响因子：
3.5
通讯作者：
Iori Yamanaka;Satoshi Koinuma;Y. Shigeyoshi;Y. Uchiyama;K. Yagita
中科院分区：
文献类型：
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作者：
Iori Yamanaka;Satoshi Koinuma;Y. Shigeyoshi;Y. Uchiyama;K. Yagita
在哺乳动物中，mCRY蛋白是必需的，并且是昼夜节律反馈回路中的主要负元件。在这项研究中，强大的生物钟振荡，即使在mCry 1的组成性过表达的条件下，在大鼠-1细胞。产生稳定表达mPer 2启动子驱动的荧光素酶报告基因的Rat-1细胞，其中mCry 1在四环素依赖性基因表达（Tet-On）系统下过表达。使用这些细胞，我们表明，在大鼠-1成纤维细胞的昼夜节律钟振荡持续时，mPD 1蛋白组成性积累在细胞核中。
In mammals, mCRY proteins are essential and are major negative elements in circadian feedback loops. In this study, robust circadian clock oscillation was present even under conditions with constitutive over-expression of mCry1 in rat-1 cells. Rat-1 cells were produced to stably express mPer2 promoter-driven luciferase reporter, in which mCry1 was overexpressed under a tetracycline-dependent gene expression (Tet-On) system. Using these cells, we show that circadian clock oscillations in rat-1 fibroblasts persist when the mCRY1 protein constitutively accumulates in the nuclei.