Inhibitory effect of eupatilin and jaceosidin isolated from Artemisia princeps in IgE-induced hypersensitivity
Inhibitory effect of eupatilin and jaceosidin isolated from Artemisia princeps in IgE-induced hypersensitivity
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DOI：
10.1016/j.intimp.2007.08.028
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发表时间：
2007-12-15
影响因子：
5.6
通讯作者：
Kim, Doniz-Hyun
中科院分区：
文献类型：
--
作者：
Lee, Seung Hoon;Bae, Eun-Ah;Kim, Doniz-Hyun
为了了解对脱颗粒和被动皮肤过敏反应 (PCA) 反应具有抑制活性的青蒿 (AP) 的抗过敏作用，通过脱粒抑制活性引导分馏分离了活性成分尤帕替林 (eupatilin) 和雅西西丁 (jaceosidin)，并研究了其抗过敏活性。这些分离的组分有效抑制由IgE-抗原复合物诱导的RBL-2H3细胞中β-己糖胺酶的释放，IC50值分别为3.4和4.5μM。 Eupatilin 和 jaceosidin 分别有效抑制 IgE-抗原复合物和化合物 48/80 诱导的 PCA 反应和抓挠行为。口服给予的杰西西丁比尤帕替林更有效地抑制PCA反应，尽管腹腔内给予的杰西西丁的PCA反应抑制活性几乎与尤帕替林相同。 Eupatilin 和 Jaceosidin 抑制 IgE-抗原复合物刺激的 RBL-2H3 细胞中 TNF-α 和 IL-4 的基因表达。 Eupatilin 和 Jaceosidin 抑制 NF-κ B 的激活。基于这些发现，Eupatilin 和 Jaceosidin 可能有助于预防 PCA 和瘙痒反应，这是 IgE 介导的代表性皮肤过敏性疾病。 (c) 2007 年 Elsevier B.V. 出版
To understand the antiallergic effect of Artemisia princeps (AP), which has been found to show inhibitory activity against degranulation and a passive cutaneous anaphylaxis (PCA) reaction, eupatilin and jaceosidin, as the active components, were isolated by degramilation-inhibitory activity-guided fractionation, with their antiallergic activity investigated. These isolated components potently inhibited the release of beta-hexosaminidase from RBL-2H3 cells induced by the IgE-antigen complex, with IC50 values of 3.4 and 4.5 mu M, respectively. Eupatilin and jaceosidin potently inhibited the PCA reaction and scratching behaviors induced by IgE- antigen complex and compound 48/80, respectively. Orally administered jaceosidin more potently inhibited the PCA reaction than that of eupatilin, although the PCA reaction-inhibitory activity of intraperitoneally administered jaceosidin was nearly the same as that of eupatilin. Eupatilin and jaceosidin inhibited the gene expressions of TNF-alpha and IL-4 in RBL-2H3 cells stimulated by IgE-antigen complex. Eupatilin and jaceosidin inhibited the activation of NF-kappa B. Based on these findings, eupatilin and jaceosidin may be useful for protection from the PCA and itching reactions, which are IgE-mediated representative skin allergic diseases. (c) 2007 Published by Elsevier B.V.