NUCLEOTIDE-SEQUENCE OF AMPICILLIN RESISTANCE GENE OF ESCHERICHIA-COLI PLASMID PBR322
NUCLEOTIDE-SEQUENCE OF AMPICILLIN RESISTANCE GENE OF ESCHERICHIA-COLI PLASMID PBR322
复制标题
DOI：
10.1073/pnas.75.8.3737
复制
发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
SUTCLIFFE, JG
中科院分区：
文献类型：
--
作者：
SUTCLIFFE, JG
测定了编码青霉素β-内酰胺酶的大肠杆菌pBR322的氨苄西林耐药基因的核苷酸序列。该基因编码286个氨基酸残基的蛋白质。前23个氨基酸可能形成分泌信号，因为它们不出现在成熟的酶中，其部分氨基酸序列是独立确定的。
The nucleotide sequence of the ampicillin resistance gene of pBR322, an E. coli plasmid that encodes a penicillin .beta.-lactamase, was determined. This gene codes for a protein of 286 amino acid residues. The first 23 amino acids presumably form a signal for secretion, because they do not appear in the mature enzyme, whose partial amino acid sequence was determined independently.