End group analysis of the cytosolic and mitochondrial fumarases from rat liver.
End group analysis of the cytosolic and mitochondrial fumarases from rat liver.
复制标题
大鼠肝脏胞质和线粒体延胡索酸酶的端基分析。
DOI：
10.1093/oxfordjournals.jbchem.a134410
复制
发表时间：
1983
影响因子：
2.7
通讯作者：
S. Tuboi
中科院分区：
文献类型：
--
作者：
K. Kobayashi;S. Tuboi
比较了细胞质和线粒体富马酸酶的一些分子特性。用羧肽酶（CPase） a鉴定了大鼠肝细胞胞浆和线粒体富马酸酶的羧基(C)端氨基酸为亮氨酸。由于用氯化丹酰法和氰酸盐法均不能鉴定胞浆和线粒体富马酸酶的氨基(N)端氨基酸，这两种富马酸酶的N端似乎被掩盖了。两种富马酸酶经过s -羧甲基化后，分别被pronase E和CPase A、B酶完全消化，并对氨基酸衍生物进行酸水解后的氨基酸进行高压纸电泳和氨基酸分析。线粒体富马酸酶和胞质富马酸酶的n端分别为焦谷氨酸和n -乙酰丙氨酸。为了详细比较两种延胡索酸酶的初级结构，每个延胡索酸酶都用精氨酸特异性蛋白酶消化或用溴化氰裂解。这些富马酸酶的酶解的电泳图谱彼此难以区分。
Some molecular properties of the cytosolic and mitochondrial fumarases were compared. The carboxyl(C)-terminal amino acid of both the cytosolic and mitochondrial fumarases of rat liver cell was identified as leucine by using carboxypeptidase (CPase) A. As the amino(N)-terminal amino acid of both the cytosolic and mitochondrial fumarases could not be identified by the dansyl chloride method or by the cyanate method, the N-termini of these two fumarases seems to be masked. Both fumarases, after S-carboxymethylation, were completely digested with pronase E and CPase A and B, and the amino acids with blocked amino group were analyzed by high voltage paper electrophoresis and amino acid analysis after acid hydrolysis of these amino acid derivatives. The N-termini of the mitochondrial and cytosolic fumarases were identified as pyroglutamic acid and N-acetylalanine, respectively. To compare the primary structures of the two fumarases in detail, each fumarase was digested with an arginine-specific protease or cleaved with cyanogen bromide. The electrophoretic profiles of the digests of these fumarases were indistinguishable from each other.