5 PSEUDOKNOTS ARE PRESENT AT THE 204 NUCLEOTIDES LONG 3' NONCODING REGION OF TOBACCO MOSAIC-VIRUS RNA
5 PSEUDOKNOTS ARE PRESENT AT THE 204 NUCLEOTIDES LONG 3' NONCODING REGION OF TOBACCO MOSAIC-VIRUS RNA
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DOI：
10.1093/nar/13.21.7673
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发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
BOSCH, L
中科院分区：
文献类型：
--
作者：
VANBELKUM, A;ABRAHAMS, JP;BOSCH, L
TMV RNA的104个核苷酸长的3'末端区域先前被证明包含两个假结结构（Rietveld et al. (1984), EMBO J. 3,2613 - 2619）。我们在此提供证据，证明在204个核苷酸长的3'非编码区中，另一个高度结构化的结构域紧邻95个核苷酸的trna样结构（Joshi et al. (1985) Nucleic Acids Res. 13, 347-354）。在化学修饰和酶解的基础上，提出了该区域三维折叠的模型，其中包含三个伪结。通过与相关的乙型肝炎病毒TMV-L、CcTMV和CGMMV的RNA序列比较，证实了这三个连续假结的存在。所涉及的六个双螺旋段的同轴堆叠产生了一个25碱基对长的准连续双螺旋。结果表明，托巴病毒rna 3'非翻译区的三维折叠在很大程度上是由5个假结的形成维持的。该区域在烟叶病毒CcTMV RNA中的组织表明，必须考虑在可氨酰化的植物病毒RNA中的重组事件。
The 104 nucleotides long 3' terminal region of TMV RNA was shown previously to contain two pseudoknotted structures (Rietveld et al. (1984), EMBO J. 3, 2613–2619). We here present evidence for the occurrence, within the 204 nucleotides long 3' noncoding region, of another highly structured domain located immediately adjacent to the tRNA-like structure of 95 nucleotides (Joshi et al. (1985) Nucleic Acids Res. 13, 347–354). A model for the three-dimensional folding of this region, containing three more pseudoknots, is proposed on the basis of chemical modification and enzymatic digestion.The existence of these three consecutive pseudoknots was supported by sequence comparisons with the RNA from the related tobamoviruses TMV-L, CcTMV and CGMMV. Coaxial stacking of the six double helical segments involved gives rise to the formation of a 25 basepair long quasi-continuous double helix. The results show that the three-dimensional folding of the 3' non-translated region of tobamoviral RNAs 1s largely maintained by the formation of five pseudoknots. The organisation of this region in the RNA of the tobamovirus CcTMV suggests that recombinational events among aminoacylatable plant viral RNAs have to be considered.