Distribution and immunophenotype of DC-SIGN-expressing cells in SIV-infected and uninfected macaques.
Distribution and immunophenotype of DC-SIGN-expressing cells in SIV-infected and uninfected macaques.
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SIV 感染和未感染猕猴中表达 DC-SIGN 的细胞的分布和免疫表型。
DOI：
10.1089/08892220260235380
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Lackner,AndrewA
中科院分区：
文献类型：
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作者：
Schwartz,AnnetteJ;Alvarez,Xavier;Lackner,AndrewA
DC-SIGN（树突状细胞特异性icam -3grabbingnonintegrin）是一种在树突状细胞（dc）上表达的外源性c型凝集素，已被认为在将HIV/SIV从粘膜表面运输到淋巴组织中起关键作用。虽然DC-SIGN表达的位置已经在有限数量的人体组织中确定，但其在恒河猴中的分布尚未确定。本研究采用免疫组织化学（IHC）和共聚焦显微镜技术对siv感染和未感染猕猴组织中dc - sign表达细胞的分布和免疫表型进行了研究。利用DC-SIGN单克隆和多克隆抗体对多种组织进行免疫组化。为了进一步确定DC-SIGN+细胞的免疫表型，我们用CD68、fasin和HLA-DR抗体进行了双重标记。在感染和未感染的猕猴中，DC-SIGN+细胞位于舌头、阴道、直肠和扁桃体的粘膜下层和固有层；然而，在任何组织的上皮内均未发现阳性细胞。DC-SIGN抗体也能标记肝脏内的库普弗细胞和脑内分散的血管周围细胞。在淋巴结内，除了皮层旁的交错网状细胞和生发中心内分散的滤泡树突状细胞外，窦状内还存在大量阳性细胞。未感染猕猴脾脏DC-SIGN+细胞分布相似，呈正弦波状、边缘区和交错状树突状细胞染色；然而，siv感染的猕猴脾脏中明显缺乏染色。DC-SIGN+细胞一致为CD68+，但fasin和HLA-DR-。粘膜组织上皮内DC-SIGN阳性细胞的缺失表明DC-SIGN在HIV/SIV的粘膜传代中没有显著作用。
DC-SIGN (dendriticcell-specificICAM-3grabbingnonintegrin), an external C-type lectin expressed on dendritic cells (DCs), has been proposed to play a pivotal role in trafficking HIV/SIV from mucosal surfaces to lymphoid tissues. Although the location of DC-SIGN expression has been established in a limited number of human tissues, its distribution in the rhesus macaque has not yet been determined. This study characterized the distribution and immunophenotype of DC-SIGN-expressing cells in SIV-infected and uninfected macaque tissues by immunohistochemistry (IHC) and confocal microscopy. IHC, using monoclonal and polyclonal antibodies against DC-SIGN, was performed on a variety of tissues. To further define the immunophenotype of DC-SIGN+cells, double-labeling with antibodies to CD68, fascin, and HLA-DR was done. In both infected and uninfected macaques, DC-SIGN+cells were located within the submucosa and lamina propria of tongue, vagina, rectum, and tonsil; however, no positive cells were present within the epithelium of any tissue. Antibodies to DC-SIGN also labeled Kupffer cells within the liver and scattered perivascular cells in the brain. Within lymph nodes, numerous positive cells were present within sinusoids in addition to cells consistent with interdigitating reticular cells in the paracortex and scattered follicular dendritic cells within germinal centers. In spleen of uninfected macaques, there was a similar distribution of DC-SIGN+cells with sinusoidal, marginal zone, and interdigitating dendritic cells staining; however, there was a marked paucity of staining in the spleens of SIV-infected macaques. DC-SIGN+cells were consistently CD68+, but fascin-and HLA-DR-. The absence of intraepithelial DC-SIGN-positive cells in mucosal tissues suggests that DC-SIGN does not play a significant role in transmucosal passage of HIV/SIV.