N-methyl-D-aspartate receptor 1 in the caudate-putamen nucleus:: Ultrastructural localization and co-expression with sorcin, a 22,000 mol. wt calcium binding protein
N-methyl-D-aspartate receptor 1 in the caudate-putamen nucleus:: Ultrastructural localization and co-expression with sorcin, a 22,000 mol. wt calcium binding protein
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DOI：
10.1016/s0306-4522(98)00440-0
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发表时间：
1999-01-01
期刊：
影响因子：
3.3
通讯作者：
Pickel, VM
中科院分区：
文献类型：
--
作者：
Gracy, KN;Clarke, CL;Pickel, VM
钙通过尾壳核中的N-甲基-D-天冬氨酸型谷氨酸受体进入对于正常的运动活动是必不可少的，但随着持续刺激和随后的细胞内钙释放，可产生细胞毒性。为了确定潜在的功能位点N-甲基-D-天冬氨酸受体激活在这一地区，我们研究了超微结构定位的R1亚基的N-甲基-D-天冬氨酸受体（NMDAR 1）在大鼠脑。此外，我们比较了NMDAR 1和sorcin的定位，22，000 mel。WT钙结合蛋白存在于某些纹状体神经元中并参与钙诱导的钙释放。在神经元质膜上的突触和非突触部位观察到NMDAR 1样免疫反应。在1514个NMDAR 1标记的轮廓中，62%为树突和树突棘，其余主要为无髓轴突和轴突终末。Sorcin-like免疫反应性存在于39%的含有NMDAR 1标记的图谱中，其中大多数（533/595）是树突和树突棘。1807 sorcin标记的配置文件，42%被确定，但是，作为小的过程，包括脊柱颈部和无髓鞘轴突或轴突终端。这些配置文件也偶尔包含NMDAR 1或显示突触或同位接触与其他NMDAR 1-免疫反应neurons. Results的这项研究表明，在尾壳核，NMDA受体的激活允许钙流入质膜网站主要是在树突sorcin可能发挥作用，在钙诱导的钙释放。Sorcin的存在下，在一些，但不是所有的含有NMDA的神经元在尾壳核有潜在的影响，已知的差异脆弱性的某些纹状体神经元兴奋性毒素。(C)1999年IBRO。出版社：Elsevier Science Ltd
Entry of calcium through N-methyl-D-aspartate-type glutamate receptors in the caudate-putamen nucleus is essential for normal motor activity, but can produce cytotoxicity with continued stimulation and subsequent release of intracellular calcium. To determine potential functional sites for N-methyl-D-aspartate receptor activation in this region, we examined the ultrastructural localization of the R1 subunit of the N-methyl-D-aspartate receptor (NMDAR1) in rat brain. In addition, we comparatively examined the localization of NMDAR1 and sorcin, a 22,000 mel. wt calcium binding protein present in certain striatal neurons and involved in calcium-induced calcium release. NMDAR1-Iike immunoreactivity was seen at synaptic and non-synaptic sites on neuronal plasma membranes. Of 1514 NMDAR1-labeled profiles, 62% were dendrites and dendritic spines and the remainder were mainly unmyelinated axons and axon terminals. Sorcin-like immunoreactivity was present in 39% of the profiles that contained NMDAR1 labeling, most (533/595) of which were dendrites and dendritic spines. Of 1807 sorcin-labeled profiles, 42% were identified, however, as small processes including spine necks and unmyelinated axons or axon terminals. These profiles also occasionally contained NMDAR1 or showed synaptic or appositional contacts with other NMDAR1-immunoreactive neurons.The results of this study suggest that in the caudate-putamen nucleus, activation of NMDA receptors permits calcium influx at plasmalemmal sites mainly on dendrites where sorcin may play a role in calcium-induced calcium release. The presence of sorcin in some, but not all NMDA-containing neurons in the caudate-putamen nucleus has potential implications for the known differential vulnerability of certain striatal neurons to excitotoxins. (C) 1999 IBRO. Published by Elsevier Science Ltd.