Optimized and scalable synthesis of magnetic nanoparticles for RNA extraction in response to developing countries' needs in the detection and control of SARS-CoV-2.
Optimized and scalable synthesis of magnetic nanoparticles for RNA extraction in response to developing countries' needs in the detection and control of SARS-CoV-2.
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针对发展中国家在检测和控制SARS-CoV-2方面的需要，优化和可扩展地合成用于RNA提取的磁性纳米颗粒。
DOI：
10.1038/s41598-020-75798-9
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发表时间：
2020-11-04
影响因子：
4.6
通讯作者：
González G
中科院分区：
文献类型：
--
作者：
Chacón-Torres JC;Reinoso C;Navas-León DG;Briceño S;González G
厄瓜多尔是受影响最严重的国家之一，拉丁美洲的2019年冠状病毒病(新冠肺炎)感染源于持续的经济危机。检测新冠肺炎最重要的方法之一是利用功能化磁性纳米颗粒(MNP)从鼻咽细胞中提取/纯化RNA的实时荧光RT-PCR法。这项技术允许在私营公司每天处理10,000个 ~ 测试，在当地大学每天处理大约数百个测试，以确保接触到广泛的人群。然而，这种方法的主要缺点是需要专门的具有强负电荷的MNP来提取病毒RNA来检测SARS-CoV-2病毒的存在。在这里，我们提供了一种简化的低成本方法，可以在100万亿毫升的溶液中生产10微克纳米颗粒，通过在短短两天内将该过程并行化10次，并允许进行 ~ 50,000新冠肺炎测试，从而扩大到一升。这种沟通有助于降低获取用于各种生物分子应用的MNP的成本，支持发展中国家的预算限制和化学品可获得性，特别是在新冠肺炎国际卫生紧急情况期间。
Ecuador is one of the most affected countries, with the coronavirus disease 2019 (COVID-19) infection, in Latin America derived from an ongoing economic crisis. One of the most important methods for COVID-19 detection is the use of techniques such as real time RT-PCR based on a previous extraction/purification of RNA procedure from nasopharyngeal cells using functionalized magnetic nanoparticles (MNP). This technique allows the processing of ~ 10,000 tests per day in private companies and around hundreds per day at local Universities guaranteeing to reach a wide range of the population. However, the main drawback of this method is the need for specialized MNP with a strong negative charge for the viral RNA extraction to detect the existence of the SARS-CoV-2 virus. Here we present a simplified low cost method to produce 10 g of nanoparticles in 100 mL of solution that was scaled to one litter by parallelizing the process 10 times in just two days and allowing for the possibility of making ~ 50,000 COVID-19 tests. This communication helps in reducing the cost of acquiring MNP for diverse biomolecular applications supporting developing country budgets constraints and chemical availability specially during the COVID-19 International Health Emergency.
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影响因子：
16.6
作者：
Vecera P;Chacón-Torres JC;Pichler T;Reich S;Soni HR;Görling A;Edelthalhammer K;Peterlik H;Hauke F;Hirsch A
通讯作者：
Hirsch A
影响因子：
13.2
作者：
Chan, Jasper Fuk-Woo;Kok, Kin-Hang;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
影响因子：
2.7
作者：
Yamaura, M;Camilo, RL;Toma, HE
通讯作者：
Toma, HE
影响因子：
29.4
作者：
Sunshine, Joel;Green, Jordon J.;Mahon, Kerry P.;Yang, Fan;Eltoukhy, Ahmed A.;Nguyen, David N.;Langer, Robert;Anderson, Daniel G.
通讯作者：
Anderson, Daniel G.
中国武汉市 138 例 2019 新型冠状病毒感染肺炎住院患者的临床特征。
影响因子：
120.7
作者：
Wang, Dawei;Hu, Bo;Peng, Zhiyong
通讯作者：
Peng, Zhiyong