Combined utilization of lipase-displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media
Combined utilization of lipase-displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media
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DOI：
10.1016/j.biortech.2012.12.020
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发表时间：
2013-02-01
影响因子：
11.4
通讯作者：
Lin, Ying
中科院分区：
文献类型：
--
作者：
Jin, Zi;Han, Shuang-Yan;Lin, Ying
展示脂肪酶的全细胞由于其低制备成本和简单的回收程序而成为高效的生物催化剂。研究了共溶剂条件下，分别展示于毕赤酵母（Pichia pastoris）全细胞上的南极假丝酵母脂肪酶B（CALB）和米黑根毛霉脂肪酶（RML）联合发酵生产生物柴油的效果。采用响应面法结合D-最优设计，以获得最佳反应条件的甲酯（ME）的合成。发现两种脂肪酶的协同作用以及使用叔丁醇和异辛烷作为共溶剂介质显着提高酯交换反应。在0.5 ~ 1的搅拌反应器中，以不同的原料进行放大反应，在最佳反应条件下，12 h内ME产率可达90%以上。此外，在20个重复批次循环后，ME产率保持在85%以上。总之，这种生物催化剂提供了一个有前途的路线，以有效的生物柴油生产。(C)2012爱思唯尔有限公司保留所有权利。
Lipase-displaying whole cells appear to be efficient biocatalysts because of their low preparation costs and simple recycling procedure. The combined utilization of Candida antarctica lipase B (CALB) and Rhizomucor miehei lipase (RML), separately displayed on Pichia pastoris whole cells, to produce biodiesel in co-solvent media was investigated. A response surface methodology incorporating a D-optimal design was employed to obtain the optimum reaction conditions for methyl ester (ME) synthesis. The synergistic effect of the two displayed lipases and the use of tert-butanol and isooctane as the co-solvent media were found to significantly improve the transesterification reaction. Scaled-up reactions using various types of feedstock were carried out in a 0.5-1 stirred reactor under optimum conditions, affording ME yields over 90% in 12 h. Moreover, the ME yields remained above 85% after 20 repeated batch cycles. In conclusion, this biocatalyst affords a promising route to efficient biodiesel production. (C) 2012 Elsevier Ltd. All rights reserved.