A Most Probable Number method (MPN) for the estimation of cell numbers of heterotrophic nitrifying bacteria in soil
A Most Probable Number method (MPN) for the estimation of cell numbers of heterotrophic nitrifying bacteria in soil
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DOI：
10.1023/a:1004243810473
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发表时间：
1998-02-01
期刊：
影响因子：
4.9
通讯作者：
von Berg, R
中科院分区：
文献类型：
--
作者：
Papen, H;von Berg, R
最可能数（MPN）的方法开发允许的第一次估计的细菌种群能够异养硝化。该方法被施加到酸性土壤的针叶林表现出硝酸盐生产。在这种土壤中，硝酸盐的生产不太可能被自养硝化菌催化，因为自养氨氧化菌从来没有被检测到，而自养亚硝酸盐氧化菌通常没有被发现在可观的细胞数量。所开发的MPN方法是基于在含有低浓度琼脂（0.1%）的复杂培养基（蛋白胨-肉提取物softagar培养基）中生长期间异养硝化细菌产生的亚硝酸盐/硝酸盐的存在/不存在的证明。浴供应的MPN试管中的生长培养物与足够的氧气和培养基中的低琼脂浓度的存在下，被认为是有利于可持续的亚硝酸盐/硝酸盐的生产。结果表明，在酸性森林土壤中，能够异养硝化的微生物种群占总好氧异养种群的重要部分。通过应用开发的MPN方法，几个细菌菌株的不同属以前没有描述执行异养硝化已被分离出的土壤，并已确定由细菌诊断试验。
A Most Probable Number (MPN) method was developed allowing for the first time estimation of populations of bacteria capable of heterotrophic nitrification. The method was applied to an acidic soil of a coniferous forest exhibiting nitrate production. In this soil nitrate production was unlikely to be catalyzed by autotrophic nitrifiers, since autotrophic ammonia oxidizers never could be detected, and autotrophic nitrite oxidizers were usually not found in appreciable cell numbers. The developed MPN method is based on the demonstration of the presence/absence of nitrite/nitrate produced by heterotrophic nitrifying bacteria during growth in a complex medium (peptone-meat-extract softagar medium) containing low concentrations of agar (0.1%). Bath the supply of the growing cultures in MPN test tubes with sufficient oxygen and the presence of low agar concentrations in the medium were found to be favourable for sustainable nitrite/nitrate production. The results demonstrate that in the acidic forest soil the microbial population capable of heterotrophic nitrification represents a significant part of the total aerobic heterotrophic population. By applying the developed MPN method, several bacterial strains of different genera not previously described to perform heterotrophic nitrification have been isolated from the soil and have been identified by bacterio-diagnostic tests.