Correction of fluorescence attenuation with depth in fibre and paper images collected by confocal laser scanning microscopy
Correction of fluorescence attenuation with depth in fibre and paper images collected by confocal laser scanning microscopy
复制标题
通过共焦激光扫描显微镜收集的纤维和纸张图像中荧光衰减随深度的校正
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
I. Parker
中科院分区：
文献类型：
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作者：
Li Xu;I. Parker
An empirical method is proposed to compensate for the attenuation of the detected fluorescence signal with depth in the images collected using a confocal laser scanning microscope. Application of this method to cross-sections of wood fibres or paper sheets is described. The method is based on fitting a curve to the experimental intensity versus depth data for each cross-section image. The compensation index is calculated from the fitted function and a compensated image is computed. The new fibre image has uniform contrast with depth, providing easier assessment of fibre cross-sections. Images of paper sheets, modified using the compensation technique, show structures to a depth of up to 80 μm including details at a depth which could not be seen in the original images.
