Experimental evolution of resistance to an antimicrobial peptide
Experimental evolution of resistance to an antimicrobial peptide
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DOI：
10.1098/rspb.2005.3301
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发表时间：
2006-01-22
影响因子：
4.7
通讯作者：
Bell, G
中科院分区：
文献类型：
--
作者：
Perron, GG;Zasloff, M;Bell, G
基于多细胞生物免疫肽抗菌特性的一类新型抗生素作为对抗耐药微生物的主要武器，正引起越来越多的关注。据称，阳离子抗菌肽利用了细菌细胞的基本特征，因此与传统抗生素相比，产生耐药性的可能性要小得多。耐药性进化遗传学的群体模型对这一说法提出了质疑。我们通过实验室的持续选择记录了阳离子抗菌肽耐药性的实验演变。在此选择实验中，当大肠杆菌和荧光假单胞菌在补充有这种抗菌肽的培养基中繁殖 600-700 代时，22/24 个大肠杆菌谱系和荧光假单胞菌独立进化出了对 pexiganan（magainin 的类似物）的可遗传耐药机制。
A novel class of antibiotics based on the antimicrobial properties of immune peptides of multicellular organisms is attracting increasing interest as a major weapon against resistant microbes. It has been claimed that cationic antimicrobial peptides exploit fundamental features of the bacterial cell so that resistance is much less likely to evolve than in the case of conventional antibiotics. Population models of the evolutionary genetics of resistance have cast doubt on this claim. We document the experimental evolution of resistance to a cationic antimicrobial peptide through continued selection in the laboratory. In this selection experiment, 22/24 lineages of Escherichia coli and Pseudomonas fluorescens independently evolved heritable mechanisms of resistance to pexiganan, an analogue of magainin, when propagated in medium supplemented with this antimicrobial peptide for 600-700 generations.