Cellular proliferation on the vitreous surface after photocoagulation.
Cellular proliferation on the vitreous surface after photocoagulation.
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DOI：
10.1007/bf00414375
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发表时间：
1969-01-01
期刊：
影响因子：
--
通讯作者：
Gloor, B P
中科院分区：
文献类型：
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作者：
Gloor, B P
本文介绍了一种兔眼玻璃体表面整体封片的制备方法。用酒精-乙醚固定和PAS染色获得最佳结果。正常玻璃体中的细胞（“透明细胞”）的分布显示出较大的偏差，其中在小带区域中的地球仪的下半部分中的细胞含量最大，在地球仪的上半部分中的赤道视网膜区域中的细胞含量最小，并且在后部区域中的含量居中。光凝后，玻璃体表面的细胞数量在术后第一天后显著增加，第15天后缓慢下降，一个月后略高于正常水平。细胞上升发生在光凝区域周围和相应的小带区域。在形态学上，75%至90%的新细胞以及光凝区域周围和小带区域中的新细胞不能与正常的“透明细胞”区分开。这一点以及沿着在这些细胞中发现的有丝分裂活动是透明细胞可能发生真实的增殖的假定证据。这对于在人类中观察到的凝固后视网膜前纤维增生的发病机制是有意义的。
A method of vitreous surface whole mount preparation in rabbits is presented. Best results have been achieved with alcohol-ether fixation and PAS staining. The distribution of cells (“hyalocytes”) in the normal vitreous demonstrates large deviations with a maximal cell content in the lower half of the globe in the zonular region, a minimal cell content in the region of the equatorial retina in the upper half of the globe and an intermediate content in the posterior region. Following photocoagulation the number of cells on the vitreous surface increases considerably after the first postoperative day and decreases slowly after the 15th day to a level slightly above normal after one month. The cell rise takes place around the area of photocoagulation and in the corresponding zonular area. Morphologically 75% to 90% of the new cells as well around the area of photocoagulation as in the zonular region cannot be differentiated from normal “hyalocytes”. This and along with the finding of mitotic activity in these cells is presumptive evidence that a real proliferation of hyalocytes may take place. This is of interest in regard to the pathogenesis of postcoagulative epiretinal fibroplasia as observed in man.