Electrophoretic properties of pituitary gonadotropins as studied by electrofocusing.
Electrophoretic properties of pituitary gonadotropins as studied by electrofocusing.
复制标题
通过电聚焦研究垂体促性腺激素的电泳特性。
DOI：
10.1210/endo-88-4-1029
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发表时间：
1971
期刊：
影响因子：
4.8
通讯作者：
Rosemary B. Ramsey
中科院分区：
文献类型：
--
作者：
Leo E. Reichert;Rosemary B. Ramsey
1.通过在由市售合成聚氨基多羧酸两性电解质（两性啉，LKB Instruments Co.）产生的pH梯度中进行电聚焦，检查各种促性腺激素的电泳特性。等电点（pi）估计的基础上紫外吸光度和/或电聚焦后的馏分的生物活性。2.牛促黄体生成激素（LH）的pi估计为9.55。牛LH亚基Cl的pi为9.36，牛LH亚基C2的pi为8.85。不能肯定这些差异是否显著。3.绵羊、猪、大鼠和兔LH（但不包括马LH）的pi估计值与牛LH的观察值相当，pH值介于8.6和10.47之间。这些值明显高于先前报道的绵羊和猪LH通过自由边界电泳测量。马LH在3.78-8.90的pH范围内显示出活性，并且在这方面是独特的。4.研究的人LH组分显然是同源的。
1. The electrophoretic properties of a variety of gonadotropins were examined by electrofocusing in pH gradients generated by commercially available synthetic polyaminopolycarboxylic acid ampholytes (ampholines, LKB Instruments Co.). Isoelectric points (pi) were estimated on the basis of ultraviolet absorbancy and/or biologic activity of fractions after electrofocusing. 2. The pi of bovine luteinizing hormone (LH) was estimated to be 9.55. The pi of bovine LH subunit Cl was 9.36 and the pi of bovine LH subunit C2 was 8.85. It is not certain that these differences are significant. 3. Estimates of the pi of ovine, porcine, rat and rabbit LH, but not equine LH, were comparable to that observed for bovine LH, falling between pH 8.6 and 10.47. These values are considerably higher than previously reported for ovine and porcine LH as measured by free boundary electrophoresis. Equine LH showed activity over the pH range 3.78–8.90 and was unique in this regard. 4. The human LH fraction studied was apparently homog...