Telomere length varies by DNA extraction method: implications for epidemiologic research.
Telomere length varies by DNA extraction method: implications for epidemiologic research.
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DOI：
10.1158/1055-9965.epi-13-0409
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发表时间：
2013-11
期刊：
影响因子：
--
通讯作者：
Boardman LA
中科院分区：
文献类型：
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作者：
Cunningham JM;Johnson RA;Litzelman K;Skinner HG;Seo S;Engelman CD;Vanderboom RJ;Kimmel GW;Gangnon RE;Riegert-Johnson DL;Baron JA;Potter JD;Haile R;Buchanan DD;Jenkins MA;Rider DN;Thibodeau SN;Petersen GM;Boardman LA
Both shorter and longer telomeres in peripheral blood leukocyte (PBL) DNA have been associated with cancer risk. However, associations remain inconsistent across studies of the same cancer type. This study compares DNA preparation methods to determine telomere length from colorectal cancer patients. We examined PBL relative telomere length (RTL) measured by quantitative PCR (qPCR) in 1,033 colorectal cancer patients and 2,952 healthy controls. DNA was extracted with Phenol/Chloroform, PureGene or QIAamp. We observed differences in RTL depending on DNA extraction method (p<0.001). Phenol/Chloroform extracted DNA had a mean RTL (T/S ratio) of 0.78 (range 0.01-6.54) ) compared to PureGene extracted DNA (mean RTL of 0.75; range 0.00-12.33). DNA extracted by QIAamp yielded a mean RTL of 0.38 (range 0.02-3.69). We subsequently compared RTL measured by qPCR from an independent set of 20 colorectal cancer cases and 24 normal controls in PBL DNA extracted by each of the three extraction methods. The range of RTL measured by qPCR from QIAamp-extracted DNA (0.17-0.58-) was smaller than from either PureGene or Phenol/Chloroform (ranges:0.04-2.67 and 0.32-2.81, respectively). RTL measured by qPCR from QIAamp-extracted DNA was smaller than from either PureGene or Phenol/Chloroform (p<0.001). Differences in DNA extraction method may contribute to the discrepancies between studies seeking to find an association between the risk of cancer or other diseases and RTL.
