degS (hhoB) is an essential Escherichia coli gene whose indispensable function is to provide σE activity
degS (hhoB) is an essential Escherichia coli gene whose indispensable function is to provide σE activity
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DOI：
10.1046/j.1365-2958.2001.02475.x
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发表时间：
2001-06-01
影响因子：
3.6
通讯作者：
Gross, CA
中科院分区：
文献类型：
--
作者：
Alba, BM;Zhong, HJ;Gross, CA
DegS（HhoB）是一种与DegP/HtrA相关的推定丝氨酸蛋白酶，调节必需的大肠杆菌sigma因子sigma（E）的基础和诱导活性，其参与细胞对细胞质外应激的反应。DegS促进sigma（E）特异性抗sigma因子RseA的去稳定化，从而释放sigma（E）以指导基因表达。我们证明degS是一个重要的E。coli基因的表达，并表明DegS的基本功能是为细胞提供sigma（E）活性。我们还表明，DegS的推定的活性位点是周质和DegS需要其N-末端跨膜结构域的sigma（E）相关的功能。
DegS (HhoB), a putative serine protease related to DegP/HtrA, regulates the basal and induced activity of the essential Escherichia coli sigma factor sigma (E), which is involved in the cellular response to extracytoplasmic stress. DegS promotes the destabilization of the sigma (E)-specific anti-sigma factor RseA, thereby releasing sigma (E) to direct gene expression. We demonstrate that degS is an essential E. coli gene and show that the essential function of DegS is to provide the cell with sigma (E) activity. We also show that the putative active site of DegS is periplasmic and that DegS requires its N-terminal transmembrane domain for its sigma (E)-related function.