Fragmentation of phosphopeptides in an ion trap mass spectrometer
Fragmentation of phosphopeptides in an ion trap mass spectrometer
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DOI：
10.1016/s1044-0305(98)00088-9
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发表时间：
1998-11-01
影响因子：
3.2
通讯作者：
Qin, J
中科院分区：
文献类型：
--
作者：
DeGnore, JP;Qin, J
本文系统地研究了电喷雾(ESI)离子陷阱质谱仪中磷肽的裂解规律。我们发现含有磷酸酪氨酸和磷酸苏氨酸的多肽呈现出复杂的碎裂模式。观察到这些磷酸肽以H3PO4和/或HPO3的形式失去了磷酸部分，但也没有检测到磷酸基团的丢失。失去部分磷酸盐的倾向强烈地依赖于电荷状态。因此，观察到的最高电荷状态倾向于沿肽骨架保留具有广泛碎裂的磷酸部分。我们还发现，含有磷酸丝氨酸的多肽具有相对简单的丢失H3PO4的碎裂模式。这种损耗与带电状态无关。我们提出了使用离子陷阱质谱仪准确识别磷酸化位点的策略。(J am Soc MS Spectrm 1998，9,1175-1188)(C)1998美国质谱学学会。
A systematic study of the fragmentation pattern of phosphopeptides in an electrospray (ESI) ion trap mass spectrometer is presented. We show that phosphotyrosine- and phosphothreonine-containing peptides show complicated fragmentation patterns. These phosphopeptides were observed to lose the phosphate moiety in the form of H3PO4 and/or HPO3, but were also detected with no loss of the phosphate group. The tendency to lose the phosphate moiety depends strongly on the charge state. Thus, the highest observed charge state tends to retain the phosphate moiety with extensive fragmentation along the peptide backbone. We also show that phosphoserine-containing peptides have relatively simple fragmentation patterns of losing H3PO4. This loss is independent of the charge state. We suggest strategies for the accurate identification of phosphorylation sites using the ion trap mass spectrometer. (J Am Soc Mass Spectrom 1998, 9, 1175-1188) (C) 1998 American Society for Mass Spectrometry.